Cloning and expression of canine interferon-alpha genes in Escherichia coli

• Published in: Journal of Veterinary Medical Science Vol. 67; no. 10; pp. 1059 - 1062
• Main Authors: Taira, O.(Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine and Animal Sciences), Watanugi, I, Hagiwara, Y, Takahashi, M, Arai, S, Sato, H, Maehara, N
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-10-2005
• Subjects: Adenoviruses, Canine; Amino Acid Sequence; Animals; canine IFN-α; Cell Line; CHIEN; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; cloning; Cloning, Molecular - methods; Cytopathogenic Effect, Viral; DNA Primers; DNA, Complementary - genetics; DOGS; Dogs - genetics; ESCHERICHIA COLI; EXPRESION GENICA; expression; EXPRESSION DES GENES; GENE EXPRESSION; INTERFERON; Interferon-alpha - genetics; INTERFERONAS; INTERFERONS; MOLECULAR CLONING; Molecular Sequence Data; PERRO; Phylogeny; Sequence Analysis, DNA; Sequence Homology; Species Specificity; Vesicular stomatitis New Jersey virus; Vesiculovirus
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.67.1059

We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1, but not against canine herpesvirus-1.