Optogenetic probing of functional brain circuitry

Recently developed optogenetic technologies offer the promise of high‐speed mapping of brain circuitry. Genetically targeted light‐gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic...

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Bibliographic Details
Published in:	Experimental physiology Vol. 96; no. 1; pp. 26 - 33
Main Authors:	Mancuso, James J., Kim, Jinsook, Lee, Soojung, Tsuda, Sachiko, Chow, Nicholas B. H., Augustine, George J.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-01-2011 John Wiley & Sons, Inc
Subjects:	Animals Brain - physiology Brain Mapping - methods Genetic Engineering - methods Light Neurons - physiology Optics and Photonics - methods Rhodopsin - physiology
Online Access:	Get full text
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Summary:	Recently developed optogenetic technologies offer the promise of high‐speed mapping of brain circuitry. Genetically targeted light‐gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic probes of neuronal activity, such as Clomeleon and Mermaid, allow light to be used to monitor the activity of a genetically defined population of neurons. Combining these two complementary sets of optogenetic probes will make it possible to perform all‐optical circuit mapping. Owing to the improved efficiency and higher speed of data acquisition, this hybrid approach should enable high‐throughput mapping of brain circuitry.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-3 ObjectType-Review-1 ObjectType-Feature-1
ISSN:	0958-0670 1469-445X
DOI:	10.1113/expphysiol.2010.055731