Importance of Circ0009910 in colorectal cancer pathogenesis as a possible regulator of miR-145 and PEAK1

• Published in: World journal of surgical oncology Vol. 19; no. 1; p. 265
• Main Authors: Kadkhoda, Sepideh, Taslimi, Reza, Noorbakhsh, Farshid, Darbeheshti, Farzaneh, Bazzaz, Javad Tavakkoly, Ghafouri-Fard, Soudeh, Shakoori, Abbas
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03-09-2021; BioMed Central; BMC
• Subjects: Bioinformatics; Biomarkers; Cancer; Cell Proliferation; Circ0009910; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - genetics; Correlation coefficient; Correlation coefficients; Cytokines; Development and progression; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; Humans; Kinases; Mathematical analysis; Metastasis; MicroRNAs - genetics; miR-145-5p; Neoplasia; Neoplasms; Non-coding RNA; Pathogenesis; Patients; PEAK1; Prognosis; Protein-Tyrosine Kinases - metabolism; Radiation therapy; RNA; RNA, Circular - genetics; Statistical analysis; Statistical methods; Thermal cycling; Tumors; Iran; Circ0009910; PEAK1; Non-coding RNA; Colorectal cancer; miR-145-5p
• ISSN: 1477-7819; 1477-7819
• DOI: 10.1186/s12957-021-02378-0

Colorectal cancer (CRC) is one of the most frequent neoplasms in the world. Based on the emerging role of noncoding RNAs, particularly circular RNAs in pathogenesis of cancers, we designed this study to inspect the expression levels of a circ0009910-mediated regulatory pathway in colorectal cancer.
After bioinformatics analyses and construction of putative circ0009910/ miR-145-5p/PEAK1 pathway, the expression levels of these components were evaluated in 50 CRC tissues and adjacent specimens by quantitative real-time PCR. Moreover, we appraised the correlation coefficients between these transcripts and calculated the correlation between circ0009910 expression levels with clinicopathological features of patients.
Circ0009910 and PEAK1 were significantly upregulated, while miR-145-5p was decreased in CRC samples compared with adjacent tissues (p < 0.05). Moreover, statistically significant correlations were observed between expression levels of circ0009910, miR-145-5p, and PEAK1. We also reported considerable correlations between circ0009910 expression and clinicopathological parameters including sex and perineural invasion. Finally, ROC curve analysis showed circ0009910 level as a discriminative biomarker for CRC.
For the first time, we could introduce circ0009910 as an important biomarker in CRC. Collectively, this investigation helped us to identify a newly diagnosed pathway in CRC that can be a potential axis for designing effective drugs for treatment of CRC patients.