Influence of transfusion of lymphokine-activated T killer cells on inflammatory responses in dogs after laparotomy

• Published in: Journal of Veterinary Medical Science Vol. 78; no. 4; pp. 579 - 585
• Main Authors: MIE, Keiichiro, TOMIHARI, Mizuki, HOSHI, Kiyotaka, NAKAMURA, Takashi, YAMAGUCHI, Tomohiro, MIYAHARA, Kazuro, SHIMADA, Terumasa
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-01-2016; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; anti-inflammatory cytokine; C-Reactive Protein - metabolism; canine; cell-mediated immune cytokine; Clinical Pathology; Dogs; inflammation; Inflammation - therapy; Inflammation - virology; Interleukins - metabolism; Killer Cells, Lymphokine-Activated - transplantation; Laparotomy - veterinary; lymphokine-activated T killer cell; Male; Natural Killer T-Cells - transplantation; Neutrophils - immunology; Postoperative Complications - therapy; Postoperative Complications - veterinary
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.15-0626

The influence of transfusion of lymphokine-activated T killer cells (T-LAK) on inflammatory responses was examined in dogs after laparotomy. Plasma C-reactive protein (CRP) level, cell numbers of peripheral blood lymphocytes (PBLs) and T lymphocyte subsets (CD3+, CD4+ and CD8+) and mRNA expression levels of cytokines including interleukin (IL)-2, IL-12, IL-4, IL-10 and transforming growth factor (TGF)-β in peripheral blood mononuclear cells (PBMCs) were measured in dogs with (T-LAK group) or without (control group) a single T-LAK administration immediately after laparotomy. The plasma CRP level initially increased and then decreased to the normal range at 7 days after laparotomy in the T-LAK group, which was earlier than in the control group. The expression level of IL-10 mRNA showed a marked postoperative increase and was significantly higher than the preoperative level on day 7 (P<0.05), whereas the level in the control group showed no clear change after laparotomy. A significant increase in IL-2 mRNA expression level in the T-LAK group was observed on day 14, which was two weeks earlier than in the control group (P<0.05). These results suggest that T-LAK therapy in dogs after laparotomy leads to earlier resolution of postoperative inflammation by production of an anti-inflammatory cytokine (IL-10) in the early phase of the postoperative period and earlier restoration of cell-mediated immunity related to cytokine production by PBMCs.