Isolation and characterization of novel reassortant H6N1 avian influenza viruses from chickens in Eastern China

• Published in: Virology journal Vol. 15; no. 1; p. 164
• Main Authors: Wu, Haibo, Yang, Fan, Liu, Fumin, Lu, Rufeng, Peng, Xiuming, Chen, Bin, Yao, Hangping, Wu, Nanping
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24-10-2018; BioMed Central; BMC
• Subjects: A549 Cells; Animals; Antigenic characteristics; Avian flu; Avian influenza; Avian influenza viruses; Chickens; Chickens - virology; China - epidemiology; Comparative analysis; Disease transmission; Distribution; Dogs; Eastern China; Epidemics; Female; Genetic aspects; Genome, Viral; Humans; Influenza A virus - genetics; Influenza A virus - immunology; Influenza A virus - isolation & purification; Influenza A virus - pathogenicity; Influenza in Birds - epidemiology; Influenza in Birds - virology; Madin Darby Canine Kidney Cells; Mice; Mice, Inbred BALB C; Mutation; Orthomyxoviridae; Pathogenicity; Phylogeny; Poultry; Reassortant; Reassortant Viruses - genetics; Reassortant Viruses - immunology; Reassortant Viruses - isolation & purification; Reassortant Viruses - pathogenicity; RNA, Viral - genetics; Subtype H6N1; Viruses; China; United States > US; Taiwan; Avian influenza viruses; Eastern China; Subtype H6N1; Chickens; Reassortant
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-018-1063-y

The H6N1 subtype of avian influenza viruses (AIVs) can infect people with an influenza-like illness; the H6N1 viruses possess the ability for zoonotic transmission from avians into mammals, and possibly pose a threat to human health.
In 2017, live poultry markets (LPMs) in Zhejiang Province were surveyed for AIVs. To better understand the genetic relationships between these strains from Eastern China and other AIVs, all gene segments of these strains were sequenced and compared with sequences available in GenBank. In this study, we analyzed the receptor-binding specificity, antigenic characteristics, and pathogenicity of these two H6N1 viruses.
In 2017, two H6N1 AIVs were isolated from chickens during surveillance for AIVs in LPMs in Eastern China. Phylogenetic analysis showed that these strains shared genetic characteristics from H6, H10, H1, and H4 AIVs found in ducks and wild birds in East Asia. These AIV strains were able to replicate in mice without prior adaptation.
In this study, we report the discovery of new strains of H6N1 viruses from chickens with novel gene reassortments. Our results suggest that these chickens play an important role generating novel reassortments in AIVs, and emphasize the need for continued surveillance of AIV strains circulating in poultry.