Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance

• Published in: BMC microbiology Vol. 19; no. 1; p. 204
• Main Authors: Shah, Chhaya, Baral, Ratna, Bartaula, Bijay, Shrestha, Lok Bahadur
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02-09-2019; BioMed Central; BMC
• Subjects: Ampicillin; Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Bacteria; Beta lactamases; Biofilms; Care and treatment; Coliforms; Cotrimoxazole; Diagnosis; Drug resistance; E coli; Escherichia coli; Females; Hemagglutinin; Hemagglutinins; Hemolysin; Infection; Lectins; Males; Mannose; MDR; Medical research; Microbial drug resistance; Monosaccharides; Norfloxacin; Pathogenicity; Pathogens; Penicillins; Strains (organisms); UPEC; Urinary tract; Urinary tract infections; Virulence; Virulence (Microbiology); Virulence factor; Virulence factors; MDR; Hemagglutinin; Hemolysin; Virulence factor; UPEC
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-019-1587-3

Escherichia coli has found to be the predominant uropathogen (50-90%) in uncomplicated, community acquired urinary tract infection (UTI). Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors, which enable the bacteria to establish UTI. The objective of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.
Out of 105 patients, 56 (53%) were females and 49 (47%) were males. The age of the patients in the study ranged from 18 years to 87 years and majority of the patients belonged to the age group 20-29 years. Virulence factor was observed in 65% (n = 69) of UPEC and 20% (n = 22) of control isolates (P = 0.0001). Haemolysin production was observed in 34(32.3%) of uroisolates and 12 (11.4%) of control strain. Similarly, 62% of UPEC and 1% of control produced biofilm (P = 0.0001). The expression of Mannose-resistant hemagglutinin (MRHA) and mannose-sensitive hemagglutinin (MSHA) in uroisolates were 52.3% (n = 55) and 5.7% (n = 6) respectively, whereas in faecal isolates, 8.5% (n = 9) expressed MRHA and none produced MSHA. Antimicrobial resistance showed a high degree of resistance towards ampicillin, cotrimoxazole and norfloxacin. The resistance was observed in significant higher degree in biofilm formers as compared to non-formers. MDR and ESBL was observed in 51 and 46% of test strains and 9 and 7.6% of control strains (P = 0.0001).
A significant association between virulence factors of UPEC and antimicrobial resistance in UPEC was present. Routine testing of these factors and co-relation with AMR is recommended. These findings will certainly help understand the pathogenicity and proper management of UTI patients, thus decreasing the improper use of antibiotics.