The burden of hepatitis B virus (HBV) infection, genotypes and drug resistance mutations in human immunodeficiency virus-positive patients in Northwest Ethiopia

• Published in: PloS one Vol. 12; no. 12; p. e0190149
• Main Authors: Deressa, Tekalign, Damtie, Debasu, Fonseca, Kevin, Gao, Shan, Abate, Ebba, Alemu, Shitaye, Aleka, Yetemwork, Swain, Mark G, van Marle, Guido, Coffin, Carla S
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27-12-2017; Public Library of Science (PLoS)
• Subjects: Analysis; Antiretroviral agents; Antiretroviral drugs; Antiretroviral therapy; Biology and life sciences; CD4 antigen; Confidence intervals; Deoxyribonucleic acid; Disease control; Disease prevention; Disease resistance; DNA; Drug resistance; Drug therapy; Epidemiology; Gastroenterology; Genetic aspects; Genetic diversity; Genotype & phenotype; Genotypes; Health aspects; Health sciences; Hepatitis; Hepatitis B; Hepatitis B surface antigen; Hepatology; Highly active antiretroviral therapy; HIV; HIV patients; Hospitals; Human immunodeficiency virus; Immunization; Immunology; Infections; Infectious diseases; Laboratories; Lamivudine; Liver; Liver cancer; Liver diseases; Medical diagnosis; Medicine; Medicine and health sciences; Microbial drug resistance; Molecular biology; Mortality; Mutation; Nevirapine; Patients; People and Places; Primers; Public health; Risk factors; Therapy; Vaccination; Viruses; Zidovudine; Ethiopia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0190149

In sub-Saharan Africa, the hepatitis B virus (HBV) and human immunodeficiency virus (HIV) infections are endemic. Although there has been great progress in HIV care, universal HBV vaccination and care is lacking. In this study, we aimed to determine the prevalence of HBV, HBV genotypes, and drug resistance mutations in dual infected cases in a cohort of HIV patients in Northwest Ethiopia.
A total of 308 HIV-1 positive patients were enrolled into the study and tested for HBsAg in plasma. In HBsAg positive samples, HBV DNA was analyzed for HBV genotype using in-house nested PCR with HBV-specific pre-core / core or surface primers, and for HBV drug resistance mutations (DRMs) in polymerase region. Odds ratio at 95% confidence interval was calculated.
Of the 308 HIV-positive subjects, 62.7% were female, median age 38 years (range 18-68, IQR: 27-49), and the median CD4 count 405 cells/μl (IQR: 75-734). Overall, 94.2% were on antiretroviral therapy (ART) frequently with combinations of Zidovudine (AZT)- Lamivudine (3TC)-Nevirapine (NVP). HBsAg was detected in 5.5% (95%CI 2.95-8.08%) of the study participants, of which the majority were infected with HBV genotype A (7A, 2E, 2D, 1C, 1 G). All HIV/HBV positive cases were on ART with anti-HBV activity (i.e., 3TC) and 3TC associated HBV DRMs (i.e., rtV173L, rtL180M, and rtM204V) were detected in 7/13 (53.8%) subjects.
In this cross-sectional study of HIV-infected individuals, we found 5.5% HBV/HIV co-infected cases. Most were receiving the first generation anti-HBV therapy with a low genetic barrier to resistance, and several carried mutations associated with anti-HBV (3TC) drug resistance. These data underscore the importance of integrating HBV screening to the HIV treatment guidelines for better management and prevention of HBV-related liver disease.