Nanoencapsulation of buriti oil (Mauritia flexuosa L.f.) in porcine gelatin enhances the antioxidant potential and improves the effect on the antibiotic activity modulation

• Published in: PloS one Vol. 17; no. 3; p. e0265649
• Main Authors: Morais, Neyna de Santos, Passos, Thaís Souza, Ramos, Gabriela Rocha, Ferreira, Victoria Azevedo Freire, Moreira, Susana Margarida Gomes, Chaves Filho, Gildácio Pereira, Barreto, Ana Paula Gomes, Leite, Pedro Ivo Palacio, Almeida, Ray Silva de, Paulo, Cícera Laura Roque, Fernandes, Rafael, Silva, Sebastião Ânderson Dantas da, Nascimento, Sara Sayonara da Cruz, de Sousa Júnior, Francisco Canindé, de Assis, Cristiane Fernandes
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18-03-2022; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Antioxidants; Antioxidants - pharmacology; Arecaceae; Biology and Life Sciences; Bromides; Carotenoids; CHO Cells; Cricetinae; Cricetulus; Cytotoxicity; E coli; Engineering and Technology; Escherichia coli; Fatty acids; Food; Fourier transforms; Gelatin; Gentamicin; Gentamicins - pharmacology; Health sciences; Laboratories; Medicine and Health Sciences; Microbial Sensitivity Tests; Microorganisms; Minimum inhibitory concentration; Modulation; Molecular biology; Nanoparticles; Nutrition; Oil; Palms; Pharmacy; Plant Oils; Properties; Pseudomonas aeruginosa; Staphylococcus aureus; Sulfonic acid; Surfactants; Swine; Toxicity; Vegetable oils; Brazil; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0265649

The present study evaluated the cytotoxicity, antioxidant potential, and antimicrobial effect on the antibiotic activity modulation of gelatin nanoparticles containing buriti oil (OPG). The cytotoxicity analysis was performed on Chinese Hamster Ovary Cells (CHO) using a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test. The antioxidant potential of buriti oil and OPG was determined by total antioxidant capacity, reducing power, and the ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) test. The modulating antimicrobial activity was evaluated by determining the minimum inhibitory concentration (MIC) concentration against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, gentamicin and norflaxacillin. The nanoformulation of OPG did not show a cytotoxic effect on CHO cells and had a higher antioxidant potential than free buriti oil (p<0.05). The combination of antibiotics with free buriti oil and OPG was more efficient in inhibiting E. coli and P. aeruginosa than isolated norfloxacillin and gentamicin (p<0.05). Regarding the inhibition of S. aureus, OPG in combination with norfloxacillin reduced MIC by 50%. Nanoencapsulation was a viable alternative to enhance functionality and adding commercial value to buriti oil.