Rosemary supplementation (Rosmarinus oficinallis L.) attenuates cardiac remodeling after myocardial infarction in rats

• Published in: PloS one Vol. 12; no. 5; p. e0177521
• Main Authors: Murino Rafacho, Bruna Paola, Portugal Dos Santos, Priscila, Gonçalves, Andréa de Freitas, Fernandes, Ana Angélica Henrique, Okoshi, Katashi, Chiuso-Minicucci, Fernanda, Azevedo, Paula S, Mamede Zornoff, Leonardo Antonio, Minicucci, Marcos Ferreira, Wang, Xiang-Dong, Rupp de Paiva, Sergio Alberto
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11-05-2017; Public Library of Science (PLoS)
• Subjects: Acids; Animals; Anti-inflammatory agents; Antioxidants; Antioxidants (Nutrients); Apoptosis; Biology and Life Sciences; Blood pressure; Blood Pressure - drug effects; Body Weight - drug effects; Care and treatment; Collagen; Coronary vessels; Cytokines; Diet; Dietary Supplements; Echocardiography; Energy metabolism; Energy Metabolism - drug effects; Euthanasia; Feeding Behavior - drug effects; Fibrosis; Food; Health aspects; Heart; Heart - drug effects; Heart - physiopathology; Heart attack; Heart attacks; Hypertrophy; Inflammation; Internal medicine; Laboratory animals; Leaves; Male; Medical schools; Medicine; Medicine and Health Sciences; Metabolism; Morbidity; Morphology; Mortality; Myocardial infarction; Myocardial Infarction - diagnostic imaging; Myocardial Infarction - drug therapy; Myocardial Infarction - physiopathology; Natural products; NF-E2-Related Factor 2 - metabolism; Nutrition research; Ostomy; Oxidative metabolism; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Rats, Wistar; Rodents; Rosemary; Rosmarinus - chemistry; Rosmarinus officinalis; Surgery; Survival Analysis; Systole - drug effects; Systolic pressure; Tumor necrosis factor-TNF; Veins & arteries; Ventricle; Ventricular Remodeling - drug effects; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0177521

Myocardial infarction (MI) is one of the leading causes of morbidity and mortality worldwide. Dietary intervention on adverse cardiac remodeling after MI has significant clinical relevance. Rosemary leaves are a natural product with antioxidant/anti-inflammatory properties, but its effect on morphology and ventricular function after MI is unknown.
To determine the effect of the dietary supplementation of rosemary leaves on cardiac remodeling after MI, male Wistar rats were divided into 6 groups after sham procedure or experimental induced MI: 1) Sham group fed standard chow (SR0, n = 23); 2) Sham group fed standard chow supplemented with 0.02% rosemary (R002) (SR002, n = 23); 3) Sham group fed standard chow supplemented with 0.2% rosemary (R02) (SR02, n = 22); 4) group submitted to MI and fed standard chow (IR0, n = 13); 5) group submitted to MI and fed standard chow supplemented with R002 (IR002, n = 8); and 6) group submitted to MI and fed standard chow supplemented with R02 (IR02, n = 9). After 3 months of the treatment, systolic pressure evaluation, echocardiography and euthanasia were performed. Left ventricular samples were evaluated for: fibrosis, cytokine levels, apoptosis, energy metabolism enzymes, and oxidative stress. Rosemary dietary supplementation attenuated cardiac remodeling by improving energy metabolism and decreasing oxidative stress. Rosemary supplementation of 0.02% improved diastolic function and reduced hypertrophy after MI. Regarding rosemary dose, 0.02% and 0.2% for rats are equivalent to 11 mg and 110 mg for humans, respectively.
Our findings support further investigations of the rosemary use as adjuvant therapy in adverse cardiac remodeling.