Sodium nitroprusside application improves morphological and physiological attributes of soybean (Glycine max L.) under salinity stress

• Published in: PloS one Vol. 16; no. 4; p. e0248207
• Main Authors: Jabeen, Zahra, Fayyaz, Hafiza Asma, Irshad, Faiza, Hussain, Nazim, Hassan, Muhammad Nadeem, Li, Junying, Rehman, Sidra, Haider, Waseem, Yasmin, Humaira, Mumtaz, Saqib, Bukhari, Syed Asad Hussain, Khalofah, Ahlam, Al-Qthanin, Rahmah N, Alsubeie, Moodi Saham
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16-04-2021; Public Library of Science (PLoS)
• Subjects: Accumulation; Agronomy; Analysis; Antioxidants - metabolism; Ascorbate Peroxidases - metabolism; Biology; Biology and Life Sciences; Botany; Cell membranes; Chlorophyll; Chlorophyll - metabolism; Computer programs; Crop science; Crops; Cytoplasm; Data analysis; Drafting software; Editing; Electronic mail; Environmental science; Enzymes; Germination; Germplasm; Glycine max; Glycine max - growth & development; Glycine max - metabolism; Growth; Homeostasis; Laboratories; Legumes; Lipid peroxidation; Malondialdehyde - metabolism; Materials science; Methodology; Morphology; Nitroprusside - metabolism; Nitroprusside - pharmacology; Photosynthesis; Photosynthesis - drug effects; Physical Sciences; Physiological aspects; Physiology; Plant Leaves - metabolism; Research facilities; Reviews; Salinity; Salinity effects; Salinity tolerance; Salt stress (Botany); Salt Stress - drug effects; Salt Stress - physiology; Salt Tolerance - drug effects; Salt Tolerance - physiology; Seed germination; Seeds; Sodium; Sodium nitroferricyanide; Sodium nitroprusside; Software; Soybean; Soybeans; Stomata; Stress, Physiological - drug effects; Superoxide Dismutase - metabolism; Tourism; Ultrastructure; Visualization; Pakistan; Saudi Arabia; Islamabad Pakistan; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0248207

Salinity is among the major abiotic stresses negatively affecting the growth and productivity of crop plants. Sodium nitroprusside (SNP) -an external nitric oxide (NO) donor- has been found effective to impart salinity tolerance to plants. Soybean (Glycine max L.) is widely cultivated around the world; however, salinity stress hampers its growth and productivity. Therefore, the current study evaluated the role of SNP in improving morphological, physiological and biochemical attributes of soybean under salinity stress. Data relating to biomass, chlorophyll and malondialdehyde (MDA) contents, activities of various antioxidant enzymes, ion content and ultrastructural analysis were collected. The SNP application ameliorated the negative effects of salinity stress to significant extent by regulating antioxidant mechanism. Root and shoot length, fresh and dry weight, chlorophyll contents, activities of various antioxidant enzymes, i.e., catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) were improved with SNP application under salinity stress compared to control treatment. Similarly, plants treated with SNP observed less damage to cell organelles of roots and leaves under salinity stress. The results revealed pivotal functions of SNP in salinity tolerance of soybean, including cell wall repair, sequestration of sodium ion in the vacuole and maintenance of normal chloroplasts with no swelling of thylakoids. Minor distortions of cell membrane and large number of starch grains indicates an increase in the photosynthetic activity. Therefore, SNP can be used as a regulator to improve the salinity tolerance of soybean in salt affected soils.