Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 14
• Main Authors: Ishihata, Hiroshi, Aoki, Makiko, Sato, Chikako, Tanaka, Masaru, Kitakami, Erika
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01-01-2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Acrylates - chemistry; Acrylates - therapeutic use; Biocompatibility; Biocompatible Materials - therapeutic use; Cell adhesion; Cell adhesion & migration; Cell Adhesion - drug effects; Cell proliferation; Cell Proliferation - drug effects; Dental research; Health aspects; Humans; Ligaments; Medical research; Methods; Periodontal Ligament - drug effects; Periodontics; Physiological aspects; Polyethylene Glycols - chemistry; Polyethylene Glycols - therapeutic use; Polyethylene Terephthalates; Polymers - chemistry; Polymers - therapeutic use; Polymers in medicine; Stem cells; Surface Properties; Tissue Engineering; Tissue Scaffolds - chemistry; Transplants & implants; Japan
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/102648

Human periodontal ligament (PDL) cells obtained from extracted teeth are a potential cell source for tissue engineering. We previously reported that poly(2-methoxyethyl acrylate) (PMEA) is highly biocompatible with human blood cells. In this study, we investigated the adhesion, morphology, and proliferation of PDL cells on PMEA and other types of polymers to design an appropriate scaffold for tissue engineering. PDL cells adhered and proliferated on all investigated polymer surfaces except for poly(2-hydroxyethyl methacrylate) and poly[(2-methacryloyloxyethyl phosphorylcholine)-co-(n-butyl methacrylate)]. The initial adhesion of the PDL cells on PMEA was comparable with that on polyethylene terephthalate (PET). In addition, the PDL cells on PMEA spread well and exhibited proliferation behavior similar to that observed on PET. In contrast, platelets hardly adhered to PMEA. PMEA is therefore expected to be an excellent scaffold for tissue engineering and for culturing tissue-derived cells in a blood-rich environment.