Anti-anaphylactic effects of Trichilia monadelpha (Thonn.) J. J. De Wilde extracts on rodent models of anaphylaxis

• Published in: Research in pharmaceutical sciences Vol. 11; no. 5; pp. 397 - 404
• Main Authors: Ben, Inemesit, Woode, Eric, Koffuor, George, Asiamah, Emmanuel
• Format: Journal Article
• Language: English
• Published: Iran Wolters Kluwer - Medknow Publications 01-01-2016; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Analysis; Anaphylaxis; Interleukins; mast cells degranulation; trichilia monadelpha; interleukin-6; tnf-α; anaphylaxis; Original; Rankings; Skin; Tumor necrosis factor; Anaphylaxis; Trichilia monadelpha; TNF-α; Mast cells degranulation; Interleukin-6
• ISSN: 1735-5362; 1735-9414
• DOI: 10.4103/1735-5362.192491

Effects of petroleum ether and ethanolic extracts of Trichilia monadelpha stem bark (PEE and EAE) on compound 48/80-induced systemic and passive anaphylaxis were determined. Survival rate, extravasation, degranulation of mast cells, and secretion of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured after pre-treatment with extracts (10-100 mg/kg) and disodium chromoglycate (2.5-250 µg/kg) and induction of anaphylaxis in C57BL/6 mice or Sprague-Dawley rats with compound 48/80. Histopathological assessments were made from skin biopsies of rats. Data was analyzed by Kaplan-Meier Survival Log-Rank Analysis, or One-way ANOVA and Holm-Sidak′s post hoc test. PEE and EAE inhibited (P ≤ 0.0001) tremors in systemic anaphylaxis passive cutaneous anaphylactic reactions and extravasation, stabilized or prevented (P ≤ 0.001-0.0001) mast cell degranulation, and inhibited (P ≤ 0.001-0.0001) TNF-α and IL-6 secretion. Per the findings, PEE and EAE of T. monadelpha have exhibited substantial anti-anaphylactic and anti-inflammatory property (with PEE performing better) which substantiates its use traditionally in management of allergies and other inflammatory disorders .