Can rapid dengue diagnostic kits be trusted? A comparative study of commercially available rapid kits for serodiagnosis of dengue fever

• Published in: Journal of laboratory physicians Vol. 11; no. 1; pp. 063 - 067
• Main Authors: Garg, Atul, Garg, Jaya, Singh, Dharam, Dhole, TN
• Format: Journal Article
• Language: English
• Published: A-12, Second Floor, Sector -2, NOIDA -201301, India Thieme Medical and Scientific Publishers Private Ltd 01-01-2019; Wolters Kluwer India Pvt. Ltd; Medknow Publications and Media Pvt. Ltd; Thieme Medical Publishers Inc; Wolters Kluwer - Medknow; Thieme Medical and Scientific Publishers Pvt. Ltd
• Subjects: Accuracy; Antibodies; Antigens; Comparative literature; dengue; Dengue fever; Disease; Enzyme-linked immunosorbent assay; Enzymes; Health aspects; igm antibodies; Illnesses; Immunoglobulins; Infection; Infections; Medical research; Medical tests; Mortality; ns1 antigen; Original; Original Article; rapid diagnostic test kits; Studies; Tropical diseases; Vectors (Biology); Virology; IgM antibodies; NS1 antigen; Dengue; rapid diagnostic test kits; enzyme-linked immunosorbent assay
• ISSN: 0974-2727; 0974-7826
• DOI: 10.4103/JLP.JLP_140_18

Abstract
BACKGROUND
: Dengue virus infection is an important emerging disease of the tropical and subtropical regions and is mainly diagnosed by serological detection of NS1 antigen and IgM antidengue antibodies. Since enzyme-linked immunosorbent assay (ELISA) facilities are not easily available at most diagnostic centers, so most of them use various commercially available rapid diagnostic tests (RDTs) kits.
AIMS AND OBJECTIVES
: This study was designed to access the diagnostic accuracy of four commercially available and widely used RDTs for serodiagnosis of dengue virus infection in Indian laboratories.
SUBJECTS AND METHODS
: The study was conducted at Department of Microbiology, G.S.V.M Medical College, Kanpur, India, to estimate the sensitivity and specificity of following RDTs: (1) Dengue Cassette (Panbio, Australia), (2) Bioline Dengue Duo (SD Diagnostics, Korea), (3) Dengue Day 1 test (J Mitra and Co., India), and (4) Dengucheck Duo (Tulip Diagnostics, India) on 72 confirmed dengue serum samples that were positive by dengue reverse transcription-polymerase chain reaction, dengue NS1, and IgM ELISA along with 80 serum samples from nondengue febrile illness patients.
RESULTS
: The majority of the RDTs demonstrated low sensitivity but good specificity for detecting NS1 antigen. Detection of antidengue IgM antibodies by RDTs demonstrated low sensitivity ranging from 27.8% to 77.7%. However, specificity was generally higher (50%–86.2%) and more consistent across the assays.
CONCLUSION
: The study results differed markedly from the RDTs manufacturers’ claimed performance characteristics. Therefore, the RDT results should be interpreted cautiously and ELISA should be performed as far as possible for serodiagnosis of dengue virus infection.