Genetic stability of RSV-F expression and the restricted growth phenotype of a live attenuated PIV3 vectored RSV vaccine candidate (MEDI-534) following restrictive growth in human lung cells

• Published in: Vaccine Vol. 31; no. 36; pp. 3756 - 3762
• Main Authors: Nelson, Christine L, Tang, Roderick S, Stillman, Elizabeth A
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 12-08-2013; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Applied microbiology; Attenuation; Biological and medical sciences; Cell Line; Cercopithecus aethiops; Children; Clinical trials; Clinical Trials, Phase I as Topic; Cricetinae; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Viral; Genetic stability; Genotype & phenotype; Hamster; Humans; Immune response; Immunization; Infections; Macaca mulatta; Mesocricetus; Microbiology; Miscellaneous; MRC-5; Mutation; Parainfluenza virus; Parainfluenza Virus 3, Bovine - genetics; Parainfluenza Virus 3, Human - genetics; Parainfluenza virus type 3 (PIV3); Pediatrics; Plasmids; Respiratory syncytial virus; Respiratory syncytial virus (RSV); Respiratory Syncytial Viruses - classification; Respiratory Syncytial Viruses - genetics; Respiratory Syncytial Viruses - immunology; Respiratory tract; RNA polymerase; RNA, Viral - genetics; Sequence Analysis, RNA; Statistical analysis; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - immunology; Vero Cells; Viral Fusion Proteins - immunology; Viral Vaccines - immunology; Virology; Virus Cultivation; Virus Replication; Viruses; Respiratory syncytial virus (RSV); Attenuation; MRC-5; Parainfluenza virus type 3 (PIV3); Genetic stability; Hamster; Human; Lung; Rodentia; Vaccine; Respiratory system; Paramyxoviridae; Virus; Paramyxovirinae; Mononegavirales; Vertebrata; Phenotype; Mammalia; Genetics; Attenuated strain; Vector
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2013.04.015

Highlights • MRC-5 cells provided restrictive growth environment for RSV vaccine candidate. • Evaluated stability of RSV-F expression and maintenance of attenuation phenotype. • Minor decline in attenuation and RSV-F expression after 23 passages in MRC-5 cells. • Modified virus still able to elicit protective anti-RSV immune responses in hamsters.