Radiological and histopathological evaluation of experimentally-induced periapical lesion in rats

• Published in: Journal of applied oral science Vol. 19; no. 5; pp. 500 - 504
• Main Authors: Teixeira, Renata Cordeiro, Rubira, Cassia Maria Fischer, Assis, Gerson Francisco, Lauris, José Roberto Pereira, Cestari, Tania Mary, Rubira-Bullen, Izabel Regina Fischer
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01-10-2011; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Animals; Bone Resorption - diagnostic imaging; Bone Resorption - pathology; Dental Pulp Necrosis - diagnostic imaging; Dental Pulp Necrosis - pathology; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Histology; Original; Periapical diseases; Periapical Diseases - diagnostic imaging; Periapical Diseases - pathology; Radiographic Image Enhancement; Radiography, Dental, Digital - methods; Radiology; Rats; Rats, Wistar; Time Factors; Radiology; Rats; Histology; Periapical diseases
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/S1678-77572011005000020

This study evaluated experimentally-induced periapical bone loss sites using digital radiographic and histopathologic parameters.
Twenty-seven Wistar rats were submitted to coronal opening of their mandibular right first molars. They were radiographed at 2, 15 and 30 days after the operative procedure by two digital radiographic storage phosphor plates (Digora®). The images were analyzed by creating a region of interest at the periapical region of each tooth (ImageJ) and registering the corresponding pixel values. After the sacrifice, the specimens were submitted to microscopic analysis in order to confirm the pulpal and periapical status of the tooth.
There was significant statistically difference between the control and test sides in all the experimental periods regarding the pixel values (two-way ANOVA; p<0.05).
The microscopic analysis proved that a periapical disease development occurred during the experimental periods with an evolution from pulpal necrosis to periapical bone resorption.