Effects of tannin-fluoride and milk-fluoride mixture on human enamel erosion from inappropriately chlorinated pool water

• Published in: Journal of Oral Science Vol. 59; no. 3; pp. 383 - 390
• Main Authors: Boonviriya, Sumalee, Tannukit, Sissada, Jitpukdeebodintra, Suwanna
• Format: Journal Article
• Language: English
• Published: Japan Nihon University School of Dentistry 2017
• Subjects: Animals; Dentistry; Electron Probe Microanalysis; enamel erosion; Fluorides - pharmacology; Halogenation; Hardness; Humans; in vitro; inappropriately chlorinated pool; Microscopy, Electron, Scanning; Milk; milk-fluoride; Surface Properties; Swimming Pools; tannin-fluoride; Tannins - pharmacology; Tooth Erosion; enamel erosion; in vitro; milk-fluoride; inappropriately chlorinated pool; tannin-fluoride
• ISSN: 1343-4934; 1880-4926
• DOI: 10.2334/josnusd.16-0582

This in vitro study aimed to investigate the efficacy of tannin-fluoride and milk-fluoride mixtures on human enamel erosion after exposure to inappropriately chlorinated pool water. Enamel specimens were immersed in swimming pool water (pH 2.7) for 30 min and in each test reagent for 4 min once a day for 60 consecutive days (group I: control, group II: tannin-fluoride, group III: milk-fluoride, group IV: tannin-fluoride before and milk-fluoride after erosive challenge, and group V: milk containing tannin-fluoride before and after erosive exposure). Surface microhardness was assessed on days 0, 30, and 60. Scanning electron microscopy (SEM) and electron probe microanalysis (EPMA) were performed after treatment of samples for 60 days. Surface microhardness of experimental groups was ranked as follows: group III > group IV-group V > group II > group I (P < 0.05). Moreover, SEM images revealed deposition of substances on erosive enamel surface after treatment with tannin-fluoride and milk-fluoride mixtures. Furthermore, EPMA profiles showed decrease of phosphorus and increase of fluoride content in groups II and IV. In conclusion, we demonstrated that treatment with fluoridated milk with or without tannin-fluoride has protective effects against enamel erosion caused by low-pH swimming pool water.