Effect of dispersants of multi-walled carbon nanotubes on cellular uptake and biological responses

• Published in: International journal of nanomedicine Vol. 6; no. default; pp. 3295 - 3307
• Main Authors: Haniu, Hisao, Saito, Naoto, Matsuda, Yoshikazu, Kim, Yoong-Ahm, Park, Ki Chul, Tsukahara, Tamotsu, Usui, Yuki, Aoki, Kaoru, Shimizu, Masayuki, Ogihara, Nobuhide, Hara, Kazuo, Takanashi, Seiji, Okamoto, Masanori, Ishigaki, Norio, Nakamura, Koichi, Kato, Hiroyuki
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Press 01-01-2011; Dove Medical Press
• Subjects: Apoptosis - drug effects; Cell Line; Cell Survival - drug effects; cytokine secretion; cytotoxicity; Diffusion; dispersant; Epithelial Cells - chemistry; Epithelial Cells - cytology; Epithelial Cells - drug effects; Humans; intracellular concentration; Materials Testing; multi-walled carbon nanotube; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - toxicity; Nanotubes, Carbon - ultrastructure; Original Research; Particle Size; dispersant; cytokine secretion; intracellular concentration; multi-walled carbon nanotube; cytotoxicity
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/ijn.s26573

Although there have been many reports about the cytotoxicity of multi-walled carbon nanotubes (MWCNTs), the results are still controversial. To investigate one possible reason, the authors investigated the influence of MWCNT dispersants on cellular uptake and cytotoxicity. Cytotoxicity was examined (measured by alamarBlue(®) assay), as well as intracellular MWCNT concentration and cytokine secretion (measured by flow cytometry) in human bronchial epithelial cells (BEAS-2B) exposed to a type of highly purified MWCNT vapor grown carbon fiber (VGCF(®), Shōwa Denkō Kabushiki-gaisha, Tokyo, Japan) in three different dispersants (gelatin, carboxylmethyl cellulose, and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine). The authors also researched the relationship between the intracellular concentration of MWCNTs and cytotoxicity by using two cell lines, BEAS-2B and MESO-1 human malignant pleural mesothelioma cells. The intracellular concentration of VGCF was different for each of the three dispersants, and the levels of cytotoxicity and inflammatory response were correlated with the intracellular concentration of VGCF. A relationship between the intracellular concentration of VGCF and cytotoxic effects was observed in both cell lines. The results indicate that dispersants affect VGCF uptake into cells and that cytotoxicity depends on the intracellular concentration of VGCF, not on the exposed dosage. Thus, toxicity appears to depend on exposure time, even at low VGCF concentrations, because VGCF is biopersistent.