Activation of Src Kinase in Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration after Acute Ischemic Renal Injury

• Published in: The American journal of pathology Vol. 163; no. 1; pp. 277 - 286
• Main Authors: Takikita-Suzuki, Mikiko, Haneda, Masakazu, Sasahara, Masakiyo, Owada, M. Koji, Nakagawa, Takahiko, Isono, Motohide, Takikita, Shoichi, Koya, Daisuke, Ogasawara, Kazumasa, Kikkawa, Ryuichi
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01-07-2003; ASIP; American Society for Investigative Pathology
• Subjects: Animals; Biological and medical sciences; Creatinine - blood; Enzyme Activation; Humans; Immunohistochemistry; Kidney Tubules - cytology; Kidney Tubules - pathology; Kidney Tubules - physiology; Kidneys; Male; Medical sciences; Nephrology. Urinary tract diseases; Platelet Aggregation Inhibitors - metabolism; Proliferating Cell Nuclear Antigen - metabolism; Proto-Oncogene Proteins c-sis - metabolism; Rats; Rats, Sprague-Dawley; Receptor, Platelet-Derived Growth Factor beta - metabolism; Regeneration - physiology; Regular; Reperfusion Injury; src-Family Kinases - metabolism; Trapidil - metabolism; Urinary system involvement in other diseases. Miscellaneous; Kidney disease; Urinary system disease; Enzyme; Transferases; Acute; Cardiovascular disease; Activation; Regeneration; Experimental disease; Platelet; Ischemia; Kinase; Animal; Platelet derived growth factor
• ISSN: 0002-9440; 1525-2191
• DOI: 10.1016/S0002-9440(10)63651-6

We previously reported that the platelet-derived growth factor B-chain (PDGF-B)/PDGF receptor (PDGFR) axis is involved in tubular regeneration after ischemia/reperfusion injury of the kidney. In the present study, we examined the activation of Src tyrosine kinase, a crucially important signaling molecule for PDGFR, and assessed the role of Src in PDGF-B-dependent renal tubular regeneration afterischemia/reperfusion injury. Immunoblot using clone 28, a monoclonal antibody specific for the active form of Src kinases, demonstrated increased active Src expression in the injured rat kidney 6 hours after reperfusion with peak activation at 12 hours.
In vitro
kinase assay confirmed increased Src activity that concurred with PDGFR-β activation as detected by the increment of receptor-phosphorylated tyrosine. Immunohistochemistry using clone 28 demonstrated that active Src was preferentially expressed in the S3 segment of the proximal tubule in reperfused kidney, where it is not normally expressed. This enhanced expression of active Src was co-localized with the increased PDGFR expression in the tubular cells that were undergoing cell proliferation cycle. Trapidil administration suppressed Src and PDGFR-β activation in the reperfused kidney and resulted in deteriorated renal function. These findings suggest that active Src participates in PDGF-B-dependent regeneration of tubular cells from acute ischemic injury.