Models of Human Metastatic Colon Cancer in Nude Mice Orthotopically Constructed by Using Histologically Intact Patient Specimens

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 20; pp. 9345 - 9349
• Main Authors: Fu, Xinyu, Besterman, Jeffrey M., Monosov, Ann, Hoffman, Robert M.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15-10-1991; National Acad Sciences
• Subjects: Abdominal Neoplasms - pathology; Abdominal Neoplasms - secondary; Absorbable gelatin sponges; Animal models; Animals; Biological and medical sciences; Cancer; Cecum; Colonic Neoplasms - pathology; Colonic Neoplasms - surgery; Female; Humans; Intestines; Liver; Liver Neoplasms - pathology; Liver Neoplasms - secondary; Lymphatic Metastasis; Male; Medical sciences; Metastasis; Mice; Mice, Nude; Neoplasm Metastasis; Neoplasm Transplantation; Specimens; Transplantability; Transplantation, Heterologous; Tumor cell; Tumors; Human; Animal model; Growth; Rodentia; Metastasis; Heterotransplantation; Abdomen; Vertebrata; Mammalia; Mouse; Digestive diseases; Tumor; Colon
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.20.9345

There is an important need for clinically relevant animal models for human cancers. Toward this goal, histologically intact human colon-cancer specimens derived surgically from patients were implanted orthotopically to the colon or cecum of nude mice. We have observed extensive orthotopic growth in 13 of 20 cases of implanted patient colon tumors. These showed various growth patterns with subsequent regional, lymph-node, and liver metastasis, as well as general abdominal carcinomatosis. Thus, models for human colon cancer have been developed that show (i) local growth, (ii) abdominal metastasis, (iii) general abdominal carcinomatosis with extensive peritoneal seeding, (iv) lymph-node metastasis, (v) liver metastasis, and (vi) colonic obstruction. These models permit the passage of the tumors to form large cohorts. They will facilitate research into the biology of colon cancer metastatic capability and the development of new drugs active against metastatic cancer. These models may also predict the clinical course and the in vivo response to drugs of the cancer of individual patients.