Development and characterization of the α3β4α5 nicotinic receptor cellular membrane affinity chromatography column and its application for on line screening of plant extracts

• Published in: Journal of Chromatography A Vol. 1431; pp. 138 - 144
• Main Authors: Ciesla, L., Okine, M., Rosenberg, A., Dossou, K.S.S., Toll, L., Wainer, I.W., Moaddel, R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 29-01-2016
• Subjects: Alkaloids; Alkaloids - chemistry; Anabasine; Anabasine - chemistry; Binding Sites; Chemistry Techniques, Analytical - methods; Chromatography, Affinity; Fabaceae - chemistry; Frontal affinity chromatography; Lycopodiaceae - chemistry; Nicotine - analogs & derivatives; Nicotine - chemistry; Plant Extracts - chemistry; Receptors, Nicotinic - chemistry; Smoke - analysis; Subtype selectivity; Alkaloids; Frontal affinity chromatography; Anabasine; Subtype selectivity
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2015.12.065

•The α3β4α5 nicotinic receptor was fully characterized.•Anabasine is selective for the α3β4α5 versus the α3β4 nicotinic receptor.•Characterization of non-competitive inhibitor binding site by frontal chromatography.•Application to the screening of plant extracts was demonstrated.
The α3β4α5 nAChR has been recently shown to be a useful target for smoking cessation pharmacotherapies. Herein, we report on the development and characterization of the α3β4α5 nicotinic receptor column by frontal displacement chromatography. The binding affinity of the nicotine and minor alkaloids found in tobacco smoke condensates were determined for both the α3β4 and α3β4α5 nicotinic receptors. It was demonstrated that while no subtype selectivity was observed for nicotine and nornicotine, anabasine was selective for the α3β4α5 nicotinic receptor. The non-competitive inhibitor binding site was also studied and it was demonstrated while mecamylamine was not selective between subtypes, buproprion showed subtype selectivity for the α3β4 nicotinic receptor. The application of this methodology to complex mixtures was then carried out by screening aqueous-alcoholic solutions of targeted plant extracts, including Lycopodium clavatum L. (Lycopodiaceae) and Trigonella foenum graecum L. (Fabaceae) against both the α3β4 and α3β4α5 nAChRs.