Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration

• Published in: Brain research Vol. 874; no. 2; pp. 123 - 130
• Main Authors: Schmued, Larry C, Hopkins, Keri J
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 25-08-2000; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Brain - drug effects; Brain - pathology; Development. Senescence. Regeneration. Transplantation; Fluoresceins; Fluorescent Antibody Technique; Fluorescent Dyes; Fluoro-Jade B; Fundamental and applied biological sciences. Psychology; Kainic acid; Kainic Acid - pharmacology; Male; Nerve Degeneration - chemically induced; Nerve Degeneration - pathology; Neuronal degeneration; Neurons - pathology; Neuropathology; Rats; Rats, Sprague-Dawley; Staining and Labeling; Vertebrates: nervous system and sense organs; Neuronal degeneration; Cellular and molecular biology; Neuropathology; Kainic acid; Fluoro-Jade B; Vertebrata; Mammalia; Fluorescent tracer; Neuron; Rat; Rodentia; Degeneration; Localization
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(00)02513-0

Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons. This notion is supported by the conspicuous staining of degenerating neuronal elements with minimal background staining. This improved signal-to-noise ratio means that fine neuronal processes including distal dendrites, axons and axon terminals can be more readily detected and documented. Although the staining time and dye concentration are reduced, the method is as rapid, simple and reliable as the original Fluoro-Jade technique. Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques.