Inhibition of nuclear entry of HPV16 pseudovirus-packaged DNA by an anti-HPV16 L2 neutralizing antibody

• Published in: Virology (New York, N.Y.) Vol. 406; no. 2; pp. 181 - 188
• Main Authors: Ishii, Yoshiyuki, Tanaka, Keiko, Kondo, Kazunari, Takeuchi, Takamasa, Mori, Seiichiro, Kanda, Tadahito
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25-10-2010
• Subjects: 60 APPLIED LIFE SCIENCES; Active Transport, Cell Nucleus - drug effects; ANIMAL CELLS; ANIMALS; Anti-P56/75; ANTIBODIES; Antibodies, Neutralizing - pharmacology; Antibodies, Viral - pharmacology; Capsid Proteins - immunology; Cell Line; Cell Nucleus - immunology; Cell Nucleus - metabolism; Cell Nucleus - virology; DNA; DNA, Viral - genetics; DNA, Viral - metabolism; ELECTRON MICROSCOPY; ENZYMES; HELA CELLS; HPV16; Human papillomavirus 16; Human papillomavirus 16 - genetics; Human papillomavirus 16 - immunology; Human papillomavirus 16 - physiology; Humans; HYDROLASES; Infectious Disease; INHIBITION; MAMMALS; MICROORGANISMS; MICROSCOPY; Neutralizing antibody; NUCLEIC ACIDS; Oncogene Proteins, Viral - immunology; ORGANIC COMPOUNDS; Papillomavirus Infections - immunology; Papillomavirus Infections - metabolism; Papillomavirus Infections - virology; PARASITES; PEPTIDE HYDROLASES; PROTEINS; RABBITS; SERINE PROTEINASES; TRYPSIN; TUMOR CELLS; VERTEBRATES; Virus Assembly - drug effects; VIRUSES; Anti-P56/75; L2; Neutralizing antibody; HPV16
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2010.07.019

Abstract Rabbit anti-HPV16 L2 serum (anti-P56/75) neutralizes multiple oncogenic human papillomaviruses (HPVs). We inoculated HeLa cells with HPV16 pseudovirus (16PV) and with anti-P56/75-bound 16PV (16PV-Ab). Both 16PV and 16PV-Ab attached equally well to the cell surface. However, the cell-attached L1 protein of 16PV became trypsin-resistant after incubation at 37 °C, whereas approximately 20% of the cell-attached 16PV-Ab L1 remained trypsin-sensitive. Confocal microscopy of HeLa cells inoculated with 16PV revealed packaged DNA in the nucleus at 22 h after inoculation; however, nuclear DNA was not detected in cells inoculated with 16PV-Ab. Electron microscopy of HeLa cells inoculated with 16PV showed particles located in multivesicular bodies, lamellar bodies, and the cytosol after 4 h; no cytosolic particles were detected after inoculation with 16PV-Ab. These data suggest that anti-P56/75 inhibits HPV infection partly by blocking viral entry and primarily by blocking the transport of the viral genome to the nucleus.