Potential Use of Tailored Citicoline Chitosan-Coated Liposomes for Effective Wound Healing in Diabetic Rat Model

• Published in: International journal of nanomedicine Vol. 17; pp. 555 - 575
• Main Authors: Eid, Hussein M, Ali, Adel A, Ali, Ahmed M Abdelhaleem, Eissa, Essam M, Hassan, Randa M, Abo El-Ela, Fatma I, Hassan, Amira H
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01-01-2022; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Angiogenesis; Animals; Care and treatment; Chitosan; Cholesterol; citicoline; Cytidine Diphosphate Choline; Design of experiments; Diabetes; diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Efficiency; Foot diseases; Fourier transforms; Gram-positive bacteria; Humans; Hydration; Leg ulcers; Lipids; Liposomes; Medical research; Medicine, Experimental; Original Research; Rats; Soybeans; Spectrum analysis; Vascular endothelial growth factor; Wound Healing; Wounds and injuries; Egypt; Minneapolis Minnesota; United Kingdom > UK; United States > US; Germany; chitosan; wound healing; diabetes mellitus; liposomes; citicoline
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S342504

This study aimed to formulate citicoline-loaded chitosan-coated liposomes (CT-CS-LPs) for topical administration and evaluated for wound healing in a diabetic animal model.
CT-LPs were formulated via a thin-film hydration approach and coated with chitosan (CS). Box-Behnken statistical design investigated the effects of lipid amount, chitosan concentration, and cholesterol amount on vesicle diameter, surface charge, and entrapment efficiency. The potential of the optimized CT-CS-LPs gel for wound healing was further evaluated in streptozocin-induced diabetic rats. The different healing stages were evaluated by several techniques, including general and special staining techniques, in addition to antibody immunohistochemistry.
The optimized CT-CS-LPs obtained had a mean size of 211.6 nm, a 50.7% entrapment efficiency, and a positive surface charge of 32.1 mV. In addition, the optimized CT-CS-LPs exhibited in vitro sustained release behavior. The in vivo experiments revealed that treatment with the optimized CT-CS-LPs boosts the healing process of the skin wound in diabetic rats by reducing inflammation, accelerating re-epithelization, angiogenesis, fibroblast proliferation, and connective tissue remodeling, leading to rapid wound closure.
Chitosan-coated liposomes containing citicoline have emerged as a potential approach for promoting the healing process in diabetic rats. However, the therapeutic effectiveness of the suggested approach in diabetic patients needs to be investigated.