Increased abundance of Ruminococcus gnavus in gut microbiota is associated with moyamoya disease and non-moyamoya intracranial large artery disease

• Published in: Scientific reports Vol. 12; no. 1; pp. 20244 - 15
• Main Authors: Mineharu, Yohei, Nakamura, Yasuhisa, Sato, Noriaki, Kamata, Takahiko, Oichi, Yuki, Fujitani, Tomoko, Funaki, Takeshi, Okuno, Yasushi, Miyamoto, Susumu, Koizumi, Akio, Harada, Kouji H.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24-11-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 692/53/2421; 692/699/375/534; 692/699/75/593/1370; Adenosine Triphosphatases; Arteries; Cerebrovascular diseases; Feces; Gastrointestinal Microbiome - genetics; Humanities and Social Sciences; Humans; Intestinal microflora; Intracranial Arterial Diseases; Microbiota; Moyamoya disease; Moyamoya Disease - genetics; multidisciplinary; Rare Diseases; Relative abundance; RNA, Ribosomal, 16S - genetics; Roseburia; rRNA 16S; Ruminococcus; Ruminococcus - genetics; Science; Science (multidisciplinary); Ubiquitin-Protein Ligases; Vein & artery diseases
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-24496-9

Moyamoya disease (MMD) is a rare cerebrovascular disease endemic in East Asia. The p.R4810K mutation in
RNF213
gene confers a risk of MMD, but other factors remain largely unknown. We tested the association of gut microbiota with MMD. Fecal samples were collected from 27 patients with MMD, 7 patients with non-moyamoya intracranial large artery disease (ICAD) and 15 control individuals with other disorders, and 16S rRNA were sequenced. Although there was no difference in alpha diversity or beta diversity between patients with MMD and controls, the cladogram showed
Streptococcaceae
was enriched in patient samples. The relative abundance analysis demonstrated that 23 species were differentially abundant between patients with MMD and controls. Among them, increased abundance of
Ruminococcus gnavus
 > 0.003 and decreased abundance of
Roseburia inulinivorans
 < 0.002 were associated with higher risks of MMD (odds ratio 9.6,
P
 = 0.0024; odds ratio 11.1,
P
 = 0.0051). Also,
Ruminococcus gnavus
was more abundant and
Roseburia inulinivorans
was less abundant in patients with ICAD than controls (
P
 = 0.046,
P
 = 0.012). The relative abundance of
Ruminococcus gnavus
or
Roseburia inulinivorans
was not different between the p.R4810K mutant and wildtype. Our data demonstrated that gut microbiota was associated with both MMD and ICAD.