PCR-based detection and serovar identification of Salmonella in retail meat collected from wet markets in Metro Manila, Philippines

• Published in: PloS one Vol. 15; no. 9; p. e0239457
• Main Authors: Santos, Pauline Dianne M, Widmer, Kenneth W, Rivera, Windell L
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 30-09-2020; Public Library of Science (PLoS)
• Subjects: Antigens; Biology and Life Sciences; Deoxyribonucleic acid; DNA; Food contamination; Genes; Glycerol; Health surveillance; Laboratories; Meat; Medicine and Health Sciences; Methods; Pathogens; People and Places; Polymerase chain reaction; Public health; Research and Analysis Methods; Risk factors; Salmonella; Salmonella food poisoning; Serologic tests; Serology; Serotyping; Technicians; Testing; Philippines; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0239457

This study aimed to detect Salmonella from retail meat collected from nine wet markets in Metro Manila, and identify the subtypes of Salmonella isolates using molecular serotyping assays from previously developed primers. Of the 720 collected meat samples, 57.64% were found to be Salmonella-contaminated. The most predominant serogroup was Salmonella O:3, and Salmonella serogroups O:4, O:6,7, O:8, O:9, and undetermined serogroups were also found. Most frequently detected isolates in bovine meat were S. 3:e,h:1,6 (putative identity: S. Anatum) and S: 4:e,h:1,2 (putative identity: S. Saintpaul), in porcine meat was S. 3:e,h:1,6 (putative identity: S. Anatum), and S. 8:i:z6 (putative identity: S. Kentucky) was common in poultry products. This study also demonstrated retail meat samples were contaminated with multiple Salmonella serogroups and serovars. This is the first Philippine study that utilized PCR-based assays to characterize Salmonella isolates down to a serovar level and provides baseline information regarding Salmonella prevalence and serovar distribution in retail meat. Molecular serotyping performed in this study can be used as an alternative approach to traditional serotyping in surveillance of Salmonella in the Philippines since the latter is expensive, time-consuming, and requires skilled technicians.