Analysis of the Ultraviolet B Response in Primary Human Keratinocytes Using Oligonucleotide Microarrays

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 99; no. 5; pp. 2965 - 2970
• Main Authors: Sesto, Angela, Navarro, Manuel, Burslem, Frank, Jorcano, José L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 05-03-2002; National Acad Sciences; The National Academy of Sciences
• Subjects: 3T3 Cells; Animals; Apoptosis; Biological Sciences; Cells, Cultured; Cysteine Endopeptidases - radiation effects; DNA microarrays; Gene expression; Gene Expression - radiation effects; Gene Expression Profiling; Gene expression regulation; Gene Expression Regulation - radiation effects; Genes; Genetics; Humans; Irradiation; Keratinocytes; Keratinocytes - cytology; Keratinocytes - radiation effects; Mice; Multienzyme Complexes - radiation effects; Oligonucleotide Array Sequence Analysis; oligonucleotide microarrays; Oligonucleotides; Proteasome Endopeptidase Complex; Protein Biosynthesis - radiation effects; Proteins - genetics; Repression; RNA Splicing - radiation effects; RNA, Messenger; Skin; Skin cancer; Transcription, Genetic; Ultraviolet radiation; Ultraviolet Rays; Up regulation
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.052678999

UV radiation is the most important environmental skin aggressor, causing cancer and other problems. This paper reports the use of oligonucleotide microarray technology to determine changes in gene expression in human keratinocytes after UVB treatment. Examination of the effects of different doses at different times after irradiation gave a global picture of the keratinocyte response to this type of insult. Five hundred thirty-nine regulated transcripts were found and organized into nine different clusters depending on behavior patterns. Classification of these genes into 23 functional categories revealed that several biological processes are globally affected by UVB. In addition to confirming a majority up-regulation of the transcripts related to the UV-specific inflammatory and stress responses, significant increases were seen in the expression of genes involved in basal transcription, splicing, and translation as well as in the proteasome-mediated degradation category. On the other hand, those transcripts belonging to the metabolism and adhesion categories were strongly down-regulated. These results demonstrate the complexity of the transcriptional profile of the UVB response, describe several cellular processes previously not known to be affected by UV irradiation, and serve as a basis for the global characterization of UV-regulated genes and pathways.