Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model

• Published in: Vaccine Vol. 33; no. 15; pp. 1865 - 1872
• Main Authors: Pan, Qing, Pais, Roshan, Ohandjo, Adaugo, He, Cheng, He, Qing, Omosun, Yusuf, Igietseme, J.U, Eko, F.O
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 08-04-2015; Elsevier Limited
• Subjects: Abortion; Adjuvants, Immunologic; Administration, Intranasal; Allergy and Immunology; Animal diseases; Animals; Antibodies, Bacterial - immunology; Bacteria; Bacterial Vaccines - immunology; Chlamydia; Chlamydia - immunology; Chlamydia abortus; Chlamydia Infections - immunology; Chlamydia Infections - microbiology; Chlamydia Infections - prevention & control; Cloning; Cross Protection; Cytokines; Dendritic cells; Dendritic Cells - immunology; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Female; Immunity; Immunity, Innate; Immunization; Infections; Kinases; Ligands; Mice; Mice, Inbred C57BL; Oligodeoxyribonucleotides - immunology; Pmp18D; Proteins; Sexually transmitted diseases; STD; Studies; T-Lymphocytes - immunology; Toll-Like Receptors - immunology; Vaccine delivery; Vaccines; Vaccines, Subunit - administration & dosage; Vaccines, Subunit - immunology; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - immunology; Vagina - microbiology; Vibrio cholerae; Vibrio cholerae - immunology; Vibrio cholerae - isolation & purification; Zoonoses; Chlamydia abortus; Pmp18D; Vaccine delivery; Immunity; Cross protection
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2015.02.007

Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.