Identification of Proacrosin Binding Protein sp32 Precursor as a Human Cancer/Testis Antigen

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 98; no. 6; pp. 3282 - 3287
• Main Authors: Ono, Toshiro, Kurashige, Takushi, Harada, Naoki, Noguchi, Yuji, Saika, Takashi, Niikawa, Norio, Aoe, Motoi, Nakamura, Shinichiro, Higashi, Toshihiro, Hiraki, Akio, Wada, Hisashi, Kumon, Hiromi, Old, Lloyd J., Nakayama, Eiichi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 13-03-2001; National Acad Sciences; The National Academy of Sciences
• Subjects: Amino Acid Sequence; Animals; Antibodies; Antibodies, Neoplasm - blood; Antibodies, Neoplasm - immunology; Antigens; Antigens, Neoplasm - genetics; Antigens, Neoplasm - immunology; Base Sequence; Biological Sciences; Blotting, Southern - methods; Cancer; Carrier Proteins - genetics; Carrier Proteins - immunology; chromosome 12; Chromosome Mapping - methods; Chromosomes, Human, Pair 12; Complementary DNA; DNA, Complementary; Female; Gene Expression Profiling; Humans; Immunology; Male; Messenger RNA; Mice; Molecular Sequence Data; Neoplasms - genetics; Neoplasms - immunology; OY-TES-1 antigen; Polymerase chain reaction; proacrosin-binding protein; Protein precursors; Protein Precursors - genetics; Protein Precursors - immunology; Proteins; Sequence Homology, Amino Acid; sp32 protein; Swine; Testes; Testis - immunology; Tumors; Urinary bladder
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.041625098

Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans.