Phylogenetic comparison of two polycyclic aromatic hydrocarbon-degrading mycobacteria

• Published in: Applied and Environmental Microbiology Vol. 61; no. 9; pp. 3221 - 3226
• Main Authors: MEERA GOVINDASWAMI, FELDHAKE, D. J, KINKLE, B. K, MINDELL, D. P, LOPER, J. C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-09-1995
• Subjects: adn; anthropogenic soils; arn ribosomal; arn ribosomial; aromatic hydrocarbons; Bacteria; Base Sequence; Biochemistry; biodegradacion; biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; dna; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; filogenia; flora del suelo; flore du sol; Fundamental and applied biological sciences. Psychology; gene; genes; Genetics; hidrocarburos; hidrocarburos aromaticos; hydrocarbons; hydrocarbure; hydrocarbure aromatique; Isolation and description; Mission oriented research; Molecular Sequence Data; mycobacterium; Mycobacterium - genetics; Mycobacterium - growth & development; Mycobacterium - metabolism; Nucleic Acid Conformation; nucleotide sequence; phylogenie; Phylogeny; pollution du sol; polucion del suelo; Polycyclic Aromatic Hydrocarbons - metabolism; ribosomal rna; ribosomas; ribosome; ribosomes; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; secuencia nucleica; sequence nucleique; soil flora; Soil Pollutants - metabolism; soil pollution; sol anthropogene; Species Specificity; suelos antropogenicos; Biodegradation; Mycobacterium; Microorganism growth; Hydrocarbon; Nucleotide sequence; Ribosomal DNA; Phylogeny; Polycyclic aromatic compound; Characterization; Pollutant; Decontamination; Mycobacteriales; Morphology; Fingerprint method; Mycobacteriaceae; 16S-RNA; Bacteria; Isolate; Actinomycetes; Application; Comparative study
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.61.9.3221-3226.1995

Two mycobacterial strains previously isolated from fossil-fuel-contaminated environments and shown to degrade four- and/or five-ring polycyclic aromatic hydrocarbons were further characterized. The two strains, PYR-I and RJGII-135, had similar growth characteristics, colony morphologies, and scotochromogenic pigmentations. DNA amplification fingerprints obtained with total genomic DNA indicated some strain similarities but with several distinctly different bands. Moreover, phylogenetic analysis based upon essentially full-length 16S rRNA gene sequences separates the two strains as distinct species within the fast-growing group of mycobacteria. Although both strains are thermosensitive, strain PYR-I has the bulged U between positions 184 and 193 characteristic of thermotolerant mycobacteria. Both strains are of potential use for reintroduction into and bioremediation of polycyclic aromatic hydrocarbon-contaminated soils.