Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO

• Published in: Applied and Environmental Microbiology Vol. 60; no. 8; pp. 2916 - 2923
• Main Authors: Jarausch, W, Saillard, C, Dosba, F, Bove, J.M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-08-1994
• Subjects: ADN; Agronomy. Soil science and plant productions; AMPLIFICATION CHAINE POLYMERASE; Bacterial plant pathogens; Bacteriology; Base Sequence; Biological and medical sciences; Cellular biology; Chromosomes, Bacterial; Deoxyribonucleic acid; Disease; DNA; DNA Primers - genetics; DNA, Bacterial - genetics; Ecology, environment; EUROPA OCCIDENTAL; Europe; EUROPE OCCIDENTALE; FRANCE; FRANCIA; Fruit - microbiology; Fruits; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; IDENTIFICACION; IDENTIFICATION; Life Sciences; MALUS PUMILA; MICOPLASMOSIS; Microbiology; MLO; Molecular Sequence Data; Mycoplasma - classification; Mycoplasma - genetics; Mycoplasma - isolation & purification; MYCOPLASMOSE; ORGANISMOS MICOPLASMICOS; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; POLIMORFISMO; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; POLYMORPHISME; PRUNUS; PYRUS COMMUNIS; REACCION DE CADENAS DE POLIMERASA; SECUENCIA NUCLEICA; Sequence Analysis, DNA; SEQUENCE NUCLEIQUE; Tenericutes - classification; Tenericutes - genetics; Tenericutes - isolation & purification; Trees; Trees - microbiology; Europe; Plant pathogen; Rosaceae; Pyrus; Vegetal microorganism relation; Method; Mollicutes; Amplification; Restriction fragment length polymorphism; Dicotyledones; DNA; Angiospermae; Prunus; Bacteria; Spermatophyta; Molecular probe; Fruit tree; Detection; Malus; MLO; POLYMORPHISME DE RESTRICTION; AMPLIFICATION CHAINE POLYMERASE
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.60.8.2916-2923.1994

A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide sequence four primer pairs for PCR were chosen to specifically amplify DNA from MLOs associated with European diseases of fruit trees. Primer pairs specific for (i) Malus-affecting MLOs, (ii) Malus- and Prunus-affecting MLOs, and (iii) Malus-, Prunus-, and Pyrus-affecting MLOs were obtained. Restriction enzyme analysis of the amplification products revealed restriction fragment length polymorphisms between Malus-, Prunus, and Pyrus-affecting MLOs as well as between different isolates of the apple proliferation MLO. No amplification with either primer pair could be obtained with DNA from 12 different MLOs experimentally maintained in periwinkle