基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究
通过对银/祐纳米簇（Ag/PtNCs)的表面修饰调控其催化活性，建立了一种髙灵敏的比色法检测Cu2+。巯基丙酸能够抑制Ag/PtNCs的催化活性，而巯基丙酸与Cu2+作用后，将导致上述抑制作用减弱。基于上述原理，通过测量Ag/PtNCs催化TMB-H2O2反应产生的显色信号，可以实现Cu2+的比色检测。本方法检测Cu2+的线性范围为10~100nmo^L，检出限(3滓）为5.0nmo^L。将本方法应用于实际水样中Cu2+的检测，结果表明，本方法具有操作简单、成本低、灵敏度髙、特异性好等优点。...
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| Published in: | 分析化学 Vol. 45; no. 4; pp. 471 - 476 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | Chinese |
| Published: |
江南大学,食品科学与技术国家重点实验室,食品学院,无锡 214122%扬州出入境检验检疫局,扬州,225000
2017
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | 通过对银/祐纳米簇（Ag/PtNCs)的表面修饰调控其催化活性，建立了一种髙灵敏的比色法检测Cu2+。巯基丙酸能够抑制Ag/PtNCs的催化活性，而巯基丙酸与Cu2+作用后，将导致上述抑制作用减弱。基于上述原理，通过测量Ag/PtNCs催化TMB-H2O2反应产生的显色信号，可以实现Cu2+的比色检测。本方法检测Cu2+的线性范围为10~100nmo^L，检出限(3滓）为5.0nmo^L。将本方法应用于实际水样中Cu2+的检测，结果表明，本方法具有操作简单、成本低、灵敏度髙、特异性好等优点。 |
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| Bibliography: | Silver/platinum nanoclusters; Colorimetric detection; 3-Mercaptopropionic acid; Copper ions 22-1125/O6 A sensitive colorimetric method for the detection of copper ions(Cu2+)was developed based on thesurface modification of silver/platinum nanoclusters(Ag/Pt NCs)and regulation of peroxidase-like activity.Itwas found that3-mercaptopropionic acid(MPA)could inhibit the catalytic ability of Ag/Pt NCs;however,itlost the inhibition toward catalytic ability of A^^Pt NCs after oxidized by oxygen through the catalysis of Cu2+.On the basis of this,a colorimetric method was developed for the detection of Cu2+through measuring thecolorimetric signal variation of the TMB-H2O2reaction.This method exhibited high sensitivity and selectivitytoward Cu2+over a panel of other metal ions.The linear range was10-100nm ol/L and the detection lim it was5.0nm ol/L(3滓).The above method was also applied to detect real water samples and spiked samples,andthe results demonstrated that this method was simple with low cost. |
| ISSN: | 0253-3820 |
| DOI: | 10.11895/j.issn.0253-3820.160710 |