Polymerase chain reaction detection of genes responsible for multiple antibiotic resistance Staphylococcus aureus isolated from food of animal origin in Egypt

• Published in: Veterinary World Vol. 10; no. 10; pp. 1205 - 1211
• Main Authors: Seedy, Fawzy R El, Samy, A A, Salam, Hala S H, Khairy, Eman A, Koraney, Aya A
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01-10-2017
• Subjects: Antibiotic resistance; Antibiotics; Contamination; Drug resistance; Erythromycin; Food contamination; food of animal origin; Genetic aspects; Health aspects; Health risks; Kanamycin; Meat; Microbial drug resistance; Multidrug resistance; multiple antibiotic resistance; Penicillin; Polymerase chain reaction; Public health; resistance genes; Staphylococcus aureus; Egypt; resistance genes; multiple antibiotic resistance; food of animal origin; Staphylococcus aureus; polymerase chain reaction
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2017.1205-1211

The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance
isolated from food of animal origin in Egypt.
A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The
isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.
Out of 125 samples, 19
isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes
, (
, and
,
, and
. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.
Contaminated foods of animal origin may represent a source of MDR
that can be a major threat to public health.