Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101

• Published in: Bioprocess and biosystems engineering Vol. 37; no. 11; pp. 2199 - 2204
• Main Authors: Choi, Sun-A, Jung, Joo-Young, Kim, Kyochan, Kwon, Jong-Hee, Lee, Jin-Suk, Kim, Seung Wook, Park, Ji-Yeon, Yang, Ji-Won
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2014; Springer Nature B.V
• Subjects: Acids; Bioengineering; Biofuels; Biotechnology; Chemistry; Chemistry and Materials Science; Chlorides; Docosahexaenoic Acids - analysis; Environmental Engineering/Biotechnology; Extraction processes; Fatty acids; Fatty Acids - analysis; Ferric Compounds; Food Microbiology; Food Science; Imidazoles; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Ions; Lipids; Lipids - isolation & purification; Liquids; Microalgae - chemistry; Original Paper; Salts; Solvents; Stramenopiles - chemistry; Synergistic effect; Molten-salt/ionic-liquid mixture; Fatty acid content; sp. KRS101; Docosahexaenoic acid; Microalgal lipids
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-014-1197-2

In this study, lipid extraction from
Aurantiochytrium
sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of
Aurantiochytrium
sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl
3
·6H
2
O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of [Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl
3
·6H
2
O was mixed with [Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl
3
·6H
2
O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90 °C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2 % of total fatty acids). Overall, lipid extraction from
Aurantiochytrium
sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions.