Tenascin-W inhibits proliferation and differentiation of preosteoblasts during endochondral bone formation

• Published in: Biochemical and biophysical research communications Vol. 356; no. 4; pp. 935 - 941
• Main Authors: Kimura, Hiroaki, Akiyama, Haruhiko, Nakamura, Takashi, Crombrugghe, Benoit de
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18-05-2007
• Subjects: 60 APPLIED LIFE SCIENCES; ADULTS; Animals; Bmp; Bone Development - drug effects; Bone Development - physiology; BONE FRACTURES; BONE MARROW CELLS; BONE TISSUES; BORON PHOSPHIDES; Cell Differentiation - physiology; CELL PROLIFERATION; Embryonic Stem Cells - cytology; Embryonic Stem Cells - physiology; EMBRYOS; MICE; Mice, Inbred ICR; Osteoblasts - cytology; Osteoblasts - physiology; Osteogenesis; Osteogenesis - physiology; Preosteoblast; PROTEINS; SKELETON; Tenascin - metabolism; Tenascin-W; Wnt Proteins - metabolism; Zebrafish Proteins - metabolism; TN-W; Bmp; dpc; Tenascin-W; Preosteoblast; bp; Osteogenesis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.03.071

We identified a cDNA encoding mouse Tenascin-W (TN-W) upregulated by bone morphogenetic protein (Bmp)2 in ATDC5 osteo-chondroprogenitors. In adult mice,
TN-W was markedly expressed in bone. In mouse embryos, during endochondral bone formation
TN-W was localized in perichondrium/periosteum, but not in trabecular and cortical bones. During bone fracture repair, cells in the newly formed perichondrium/periosteum surrounding the cartilaginous callus expressed
TN-W. Furthermore,
TN-W was detectable in perichondrium/periosteum of
Runx2-null and
Osterix-null embryos, indicating that
TN-W is expressed in preosteoblasts. In CFU-F and -O cells, TN-W had no effect on initiation of osteogenesis of bone marrow cells, and in MC3T3-E1 osteoblastic cells TN-W inhibited cell proliferation and
Col1a1 expression. In addition, TN-W suppressed canonical Wnt signaling which stimulates osteoblastic differentiation. Our results indicate that
TN-W is a novel marker of preosteoblasts in early stage of osteogenesis, and that TN-W inhibits cell proliferation and differentiation of preosteoblasts mediated by canonical Wnt signaling.