Fungus-mediated green synthesis of nano-silver using Aspergillus sydowii and its antifungal/antiproliferative activities

• Published in: Scientific reports Vol. 11; no. 1; pp. 10356 - 9
• Main Authors: Wang, Dongyang, Xue, Baiji, Wang, Lin, Zhang, Yidi, Liu, Lijun, Zhou, Yanmin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14-05-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2234; 631/1647/350; 631/326/193; 631/326/2522; Antifungal activity; Antifungal Agents - chemical synthesis; Antifungal Agents - pharmacology; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - pharmacology; Aspergillus - chemistry; Aspergillus - metabolism; Aspergillus sydowii; Biosynthesis; Cell culture; Cell Proliferation - drug effects; Drug Screening Assays, Antitumor; Green Chemistry Technology - methods; HeLa Cells; Humanities and Social Sciences; Humans; MCF-7 Cells; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microscopy, Electron, Transmission; multidisciplinary; Nanoparticles; Science; Science (multidisciplinary); Silver; Silver - chemistry; Silver - metabolism; Silver - pharmacology; Transmission electron microscopy; X-ray diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-89854-5

Due to the increasing demand for eco-friendly, cost-effective and safe technologies, biosynthetic metal nanoparticles have attracted worldwide attention. In this study, silver nanoparticles (AgNPs) were extracellularly biosynthesized using the culture supernatants of
Aspergillus sydowii
. During synthesis, color change was preliminarily judge of the generation of AgNPs, and the UV absorption peak at 420 nm further confirms the production of AgNPs. Transmission electron microscopy and X-ray diffraction were also used to identify the AgNPs. The results shows that AgNPs has crystalline cubic feature and is a polydisperse spherical particle with size between 1 and 24 nm. Three main synthesis factors (temperature, pH and substrate concentration) were optimized, the best synthesis conditions were as follows 50 °C, 8.0 and 1.5 mM. In the biological application of AgNPs, it shows effective antifungal activity against many clinical pathogenic fungi and antiproliferative activity to HeLa cells and MCF-7 cells in vitro. Our research finds a new path to biosynthesis of AgNPs in an eco-friendly manner, and bring opportunity for biomedical applications in clinic.