A practical assessment of nano-phosphate on soybean (Glycine max) growth and microbiome establishment

• Published in: Scientific reports Vol. 10; no. 1; p. 9151
• Main Authors: McKnight, Michelle M., Qu, Zhi, Copeland, Julia K., Guttman, David S., Walker, Virginia K.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04-06-2020; Nature Publishing Group
• Subjects: 45/22; 45/23; 45/41; 45/77; 631/326/171/1818; 631/449; 639/925/357/354; 704/158/855; Bacteria - genetics; Bacteria - isolation & purification; Biomass; Durapatite - chemistry; Durapatite - pharmacology; Farm buildings; Fertilizers; Fertilizers - analysis; Glycine max; Glycine max - drug effects; Glycine max - growth & development; Glycine max - microbiology; Humanities and Social Sciences; Hydroxyapatite; Microbial activity; Microbiomes; Microbiota - drug effects; multidisciplinary; Nanoparticles - chemistry; Phosphorus; Physicochemical properties; Plant growth; Plant Roots - microbiology; Principal Component Analysis; Rhizosphere; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - metabolism; rRNA 16S; Science; Science (multidisciplinary); Soil Microbiology; Soybeans
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-66005-w

The efficacy of needle-shaped nano-hydroxyapatite (nHA; Ca
10
(PO
4
)
6
(OH)
2
) as a phosphate (Pi) fertilizer was evaluated as well as its impact on soil and soybean (
Glycine max
) bacterial and fungal communities. Microbial communities were evaluated in soy fertilized with nHA using ITS (internal transcribed spacer) and 16S rRNA high-throughput gene sequencing. Separate greenhouse growth experiments using agriculturally relevant nHA concentrations and application methods were used to assess plant growth and yield compared with no Pi (−P), soluble Pi (+P), and bulk HA controls. Overall, nHA treatments did not show significantly increased growth, biomass, total plant phosphorus concentrations, or yield compared with no Pi controls. Soil and rhizosphere community structures in controls and nHA treatment groups were similar, with minor shifts in the nHA-containing pots comparable to bulk HA controls at equal concentrations. The implementation of nHA in an agriculturally realistic manner and the resulting poor soy growth advises that contrary to some reports under specialized conditions, this nano-fertilizer may not be a viable alternative to traditional Pi fertilizers. If nano-phosphate fertilizers are to achieve their conjectured agricultural potential, alternative nHAs, with differing morphologies, physicochemical properties, and interactions with the soil matrix could be investigated using the evaluative procedures described.