Widely targeted metabolome and transcriptome landscapes of Allium fistulosum–A. cepa chromosome addition lines revealed a flavonoid hot spot on chromosome 5A

• Published in: Scientific reports Vol. 9; no. 1; p. 3541
• Main Authors: Abdelrahman, Mostafa, Hirata, Sho, Sawada, Yuji, Hirai, Masami Yokota, Sato, Shusei, Hirakawa, Hideki, Mine, Yoko, Tanaka, Keisuke, Shigyo, Masayoshi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05-03-2019; Nature Publishing Group
• Subjects: 101/58; 13; 38; 45; 45/90; 45/91; 631/208/199; 631/449/711; 64; Acyltransferase; Allium; Allium fistulosum; Biosynthesis; Chalcone synthase; Chromosomes; Drug resistance; Flavonoids; Gene expression; Genes; Glucosides; Glucosyltransferase; Humanities and Social Sciences; Hydroxylase; Liquid chromatography; Mass spectrometry; Mass spectroscopy; multidisciplinary; Naringenin 3-dioxygenase; Quercetin; Reductase; Ribonucleic acid; RNA; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-39856-1

Here, we report a comprehensive analysis of the widely targeted metabolome and transcriptome profiles of
Allium fistulosum
L. (FF) with the single extra chromosome of shallot [
A
.
cepa
L. Aggregatum group (AA)] to clarify the novel gene functions in flavonoid biosynthesis. An exhaustive metabolome analysis was performed using the selected reaction monitoring mode of liquid chromatography–tandem quadrupole mass spectrometry, revealing a specific accumulation of quercetin, anthocyanin and flavone glucosides in AA and FF5A. The addition of chromosome 5A from the shallot to
A
.
fistulosum
induced flavonoid accumulation in the recipient species, which was associated with the upregulation of several genes including the
dihydroflavonol 4-reductase
,
chalcone synthase
,
flavanone 3-hydroxylase
,
UDP-glucose flavonoid-3-O-glucosyltransferase
,
anthocyanin 5-aromatic acyltransferase-like
,
pleiotropic drug resistance-like ATP binding cassette transporter
, and
MYB14
transcriptional factor. Additionally, an open access
Allium
Transcript Database (
Allium
TDB,
http://alliumtdb.kazusa.or.jp
) was generated by using RNA-Seq data from different genetic stocks including the
A
.
fistulosum–A
.
cepa
monosomic addition lines. The functional genomic approach presented here provides an innovative means of targeting the gene responsible for flavonoid biosynthesis in
A
.
cepa
. The understanding of flavonoid compounds and biosynthesis-related genes would facilitate the development of noble
Allium
varieties with unique chemical constituents and, subsequently, improved plant stress tolerance and human health benefits.