Fine mapping of the Asian soybean rust resistance gene Rpp2 from soybean PI 230970

• Published in: Theoretical and applied genetics Vol. 128; no. 3; pp. 387 - 396
• Main Authors: Yu, Neil, Kim, Myungsik, King, Zachary R., Harris, Donna K., Buck, James W., Li, Zenglu, Diers, Brian W.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2015; Springer; Springer Nature B.V
• Subjects: Agriculture; Arabidopsis; Basidiomycota; Biochemistry; Biomedical and Life Sciences; Biotechnology; Breeding; Chromosome Mapping; Chromosomes; Cloning; Crop diseases; Disease resistance (Plants); Disease Resistance - genetics; Diseases and pests; DNA, Plant - genetics; Genes; Genes, Dominant; Genes, Plant; Genetic aspects; Genetic Markers; Genomics; Glycine max; Glycine max - genetics; Glycine max - microbiology; Haplotypes; Health aspects; Life Sciences; Methods; Microsatellite Repeats; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant introductions; Plant resistance; Polymorphism, Single Nucleotide; Rusts (Fungi); Soil sciences; Soybeans; South America; United States > US; Recombinant Plant; Rust Resistance; Plant Introduction; Single Nucleotide Polymorphism Marker; Simple Sequence Repeat Marker
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-014-2438-0

Key message
Asian soybean rust (ASR) resistance gene
Rpp2
has been fine mapped into a 188.1 kb interval on Glyma.Wm82.a2, which contains a series of plant resistance (
R
) genes.
Asian soybean rust (ASR), caused by the fungus
Phakopsora pachyrihizi
Syd. & P. Syd., is a serious disease in major soybean [
Glycine max
(L.) Merr.] production countries worldwide and causes yield losses up to 75 %. Defining the exact chromosomal position of ASR resistance genes is critical for improving the effectiveness of marker-assisted selection (MAS) for resistance and for cloning these genes. The objective of this study was to fine map the ASR resistance gene
Rpp2
from the plant introduction (PI) 230970.
Rpp2
was previously mapped within a 12.9-cM interval on soybean chromosome 16. The fine mapping was initiated by identifying recombination events in F
2
and F
3
plants using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers that flank the gene. Seventeen recombinant plants were identified and then tested with additional genetic markers saturating the gene region to localize the positions of each recombination. The progeny of these selected plants were tested for resistance to ASR and with SSR markers resulting in the mapping of
Rpp2
to a 188.1 kb interval on the Williams 82 reference genome (Glyma.Wm82.a2). Twelve genes including ten toll/interleukin-1 receptor (TIR)-nucleotide-binding site (NBS)-leucine-rich repeat (LRR) genes were predicted to exist in this interval on the Glyma.Wm82.a2.v1 gene model map. Eight of these ten genes were homologous to the Arabidopsis TIR-NBS-LRR gene
AT5G17680.1
. The identified SSR and SNP markers close to
Rpp2
and the candidate gene information presented in this study will be significant resources for MAS and gene cloning research.