Irisin Protects Against Hind Limb Ischemia Reperfusion Injury

• Published in: Drug design, development and therapy Vol. 15; pp. 361 - 368
• Main Authors: Küçük, Ayşegül, Polat, Yücel, Kılıçarslan, Aydan, Süngü, Nuran, Kartal, Hakan, Dursun, Ali Doğan, Arslan, Mustafa
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01-01-2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Analysis; Animal models; Animals; Antioxidants; Apoptosis; Caspase-3; Caspase-8; Dose-Response Relationship, Drug; Enzymes; Fibronectins - administration & dosage; Fibronectins - metabolism; Free radicals; Hindlimb - drug effects; Hindlimb - metabolism; Homogenization; Inflammation; Injections, Intraperitoneal; irisin; Ischemia; ischemia reperfusion; Laparotomy; Mice; Molecular Structure; Muscles; Original Research; Oxidants; Oxidizing agents; Protective Agents - administration & dosage; Protective Agents - pharmacology; Reagents; Reperfusion; Reperfusion injury; Reperfusion Injury - drug therapy; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Skeletal muscle; Statistical analysis; Statistical methods; Structure-Activity Relationship; Turkey; ischemia reperfusion; caspase-8; irisin; mice; caspase-3
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/DDDT.S279318

The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R).
The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I-I/R). Irisin (0.5 µg.g
, intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal-Wallis and Mann-Whitney
-tests.
There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I-I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase-3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I-I/R groups as compared with those in the control and irisin groups.
Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.