Expression analysis of four peroxiredoxin genes from Tamarix hispida in response to different abiotic stresses and Exogenous Abscisic Acid (ABA)

• Published in: International journal of molecular sciences Vol. 13; no. 3; pp. 3751 - 3764
• Main Authors: Gao, Caiqiu, Zhang, Kaimin, Yang, Guiyan, Wang, Yucheng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-03-2012; Molecular Diversity Preservation International (MDPI)
• Subjects: ABA; Abiotic stress; abiotic stresses; Abscisic acid; Abscisic Acid - pharmacology; Amino Acid Sequence; Amino acids; Antioxidants; Cadmium Chloride - pharmacology; Cloning; Cloning, Molecular; Enzymes; gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Gene regulation; Genes; Heat; High temperature; Leaves; Molecular Sequence Data; Molecular weight; Oxidative stress; Peptides; peroxide; Peroxiredoxin; Peroxiredoxins - biosynthesis; Peroxiredoxins - genetics; Phylogenetics; Plant Leaves - metabolism; Plant Roots - metabolism; Plant Stems - metabolism; Polyethylene glycol; Polyethylene Glycols - pharmacology; Polymerase chain reaction; Protein Sorting Signals - genetics; Proteins; Prx gene; Rape plants; Roots; Sequence Alignment; Signal transduction; Sodium Bicarbonate - pharmacology; Sodium chloride; Sodium Chloride - pharmacology; Stems; Stress; Stress, Physiological; Tamaricaceae - drug effects; Tamaricaceae - genetics; Tamarix; Tamarix hispida; Transcription; ABA; Tamarix hispida; abiotic stresses; Prx gene; gene expression
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms13033751

Peroxiredoxins (Prxs) are a recently discovered family of antioxidant enzymes that catalyze the reduction of peroxides and alkyl peroxides. In this study, four Prx genes (named as ThPrxII, ThPrxIIE, ThPrxIIF, and Th2CysPrx) were cloned from Tamarix hispida. Their expression profiles in response to stimulus of NaCl, NaHCO(3), PEG, CdCl(2) and abscisic acid (ABA) in roots, stems and leaves of T. hispida were investigated using real-time RT-PCR. The results showed that the four ThPrxs were all expressed in roots, stems and leaves. Furthermore, the transcript levels of ThPrxIIE and ThPrxII were the lowest and the highest, respectively, in all tissue types. All the ThPrx genes were induced by both NaCl and NaHCO(3) and reached their highest expression levels at the onset of stress in roots. Under PEG and CdCl(2) stress, the expression patterns of these ThPrxs showed temporal and spatial specificity. The expressions of the ThPrxs were all differentially regulated by ABA, indicating that they are all involved in the ABA signaling pathway. These findings reveal a complex regulation of Prxs that is dependent on the type of Prx, tissue, and the signaling molecule. The divergence of the stress-dependent transcriptional regulation of the ThPrx gene family in T. hispida may provide an essential basis for the elucidation of Prx function in future work.