Nitrobenzoates and Nitrothiobenzoates with Activity against M. tuberculosis

• Published in: Microorganisms (Basel) Vol. 11; no. 4; p. 969
• Main Authors: Pais, João P, Antoniuk, Olha, Freire, Raquel, Pires, David, Valente, Emília, Anes, Elsa, Constantino, Luis
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-04-2023; MDPI
• Subjects: Alcohol; Antibiotics; Antimicrobial activity; Antimycobacterial agents; Bacteria; Benzoates; Benzoic acid; Chloride; Chromatography; Comparative analysis; Control; Cytotoxicity; esterases; Esters; Identification and classification; Monocytes; mycobacteria; Mycobacterium tuberculosis; Nitro compounds; nitrobenzoates; Pandemics; Physiological aspects; prodrugs; Scaffolds; Sensors; Solvents; Thioesters; Toxicity; Tuberculosis; Portugal; St Louis Missouri; United States > US; nitrobenzoates; esterases; prodrugs; mycobacteria; benzoates; tuberculosis
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11040969

Esters of weak acids have shown improved antimycobacterial activity over the corresponding free acids and nitro benzoates in particular have previously shown to have a very intriguing activity. To expand the potential of nitro-derivatives of benzoic acid as antimycobacterial drugs and explore the effects of various structural features on the activity of these compounds, we have obtained a library of 64 derivatives containing esters and thioesters of benzoates and studied their activity against
, the stability of the compounds, their activation by mycobacterial enzymes and the potential cytotoxicity against human monocytic THP-1 cell line. Our results showed that the most active compounds are those with an aromatic nitro substitution, with the 3,5-dinitro esters series being the most active. Also, the greater antitubercular activity for the nitro derivatives was shown to be unrelated to their pKa values or hydrolysis rates. Given the conventional relationship between nitro-containing substances and toxicity, one might anticipate that the great antimicrobial activity of nitro compounds would be associated with high toxicity; yet, we have not found such a relationship. The nitrobenzoate scaffold, particularly the 3,5-dinitrobenzoate scaffold, merits further investigation, because it has the potential to generate future antimycobacterial agents with improved activity.