Evidence for two possible glnB-type genes in Herbaspirillum seropedicae

• Published in: Journal of Bacteriology Vol. 179; no. 14; pp. 4623 - 4626
• Main Authors: Benelli, E.M. (UFPR, Curitiba-PR, Brazil.), Souza, E.M, Funayama, S, Rigo, L.U, Pedrosa, F.O
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01-07-1997
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN RECOMBINADO; ADN RECOMBINE; AMINO ACID SEQUENCES; Ammonium Chloride - metabolism; BACTERIA FIJADORA DEL NITROGENO; BACTERIAL PROTEINS; Bacterial Proteins - genetics; BACTERIE FIXATRICE DE L'AZOTE; Bacteriology; BETA-GLUCOSIDASE; Biochemistry; CHEMICAL COMPOSITION; COMPLEMENTATION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Conjugation, Genetic; DNA Transposable Elements; ENZYMIC ACTIVITY; EXPRESION GENICA; EXPRESSION DES GENES; GENBANK/U86073; GENE; GENE EXPRESSION; Gene Expression Regulation, Bacterial; GENES; Genes, Bacterial; Genetic Complementation Test; GENETICA; GENETICS; GENETIQUE; GLNB GENE; GLUCOSIDASA; GLUCOSIDASE; GLUCOSIDASES; GLUTAMATE-AMMONIA LIGASE; Glutamate-Ammonia Ligase - metabolism; Gram-Negative Aerobic Bacteria - genetics; Gram-Negative Aerobic Bacteria - metabolism; Herbaspirillum seropedicae; KLEBSIELLA PNEUMONIAE; Klebsiella pneumoniae - genetics; LIGASAS; LIGASE; LIGASES; MICROBIAL PROTEINS; MOLECULAR SEQUENCE DATA; Mutagenesis, Insertional; MUTANT; MUTANTES; MUTANTS; Nitrates - metabolism; Nitrogen Fixation; NITROGEN FIXING BACTERIA; NUCLEOTIDE SEQUENCE; Phenotype; PII Nitrogen Regulatory Proteins; PII PROTEIN; Potassium Compounds - metabolism; PROMOTERS; PROTEINAS MICROBIANAS; PROTEINE MICROBIENNE; RECOMBINANT DNA; REPORTER GENES; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; STRUCTURAL GENES
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.179.14.4623-4626.1997

Two glnB-like genes have been isolated from Herbaspirillum seropedicae by complementation of the Klebsiella pneumoniae glnB502 mutant for growth on nitrate. One of these glnB-like genes has been sequenced and shows strong identity with GlnB proteins derived from other organisms. A Tn5-20 mutation of this glnB was Nif negative