The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt

• Published in: Veterinary World Vol. 12; no. 1; pp. 141 - 145
• Main Authors: Ibrahim, Waleed A, Marouf, Sherif A, Erfan, Ahmed M, Nasef, Soad A, Jakee, Jakeen K El
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01-01-2019
• Subjects: Ampicillin; Antibacterial agents; Antibiotic resistance; Antibiotics; Antimicrobial resistance; Bacteria; Bone marrow; Chickens; Colibacillosis; Colistin; Colistin sulfate; Diarrhea; Drug resistance; E coli; Egypt; Erythromycin; Escherichia coli; Genes; Genetic aspects; Genetic research; Labels; Levofloxacin; Liver; Lungs; Medical tests; Methylene blue; Microbial drug resistance; Omphalitis; Organs; Penicillins; Poultry; Poultry industry; qac resistance genes; Quality control; Quality management; Serotypes; Sulfamethoxazole; Sulfates; TetA gene; Tetracyclines; Trimethoprim; Yolk; Yolk sac; Egypt; chickens; antimicrobial resistance; Escherichia coli; Egypt; qac resistance genes
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2019.141-145

This work aimed to determine the occurrence of antibiotic and disinfectant resistance genes in
isolated from chickens in Egypt.
Organs (liver, lung, heart, yolk sac, and bone marrow) of 1500 chicken samples were collected from diseased chickens suffered from colibacillosis with PM findings as CRD, diarrhea and omphalitis from different governorates of Egypt as: Giza, EL-Bahira, Fayoum, El-Dakahlia, El-Ismalia, and El-Sharkia during 2015-2016. These samples were labeled and transported immediately on ice to the Reference laboratory for quality control on poultry production (RLQP). The samples were cultured onto MacConkey agar and Eosin Methylene Blue Agar. Isolation and identification of the
were performed based on morphology, cultural, staining, and biochemical properties. Antimicrobial resistance test was carried out using disk diffusion method. The PCR employing
and
were carried out for detection of these genes in isolated
.
The prevalence of
in chicken was 34%. Predominant serotypes of
which serologically identified were O128, O111, O44, O158, and O2. Antibiotic susceptibility test of
revealed that 100% of isolates were resistant to ampicillin, erythromycin, and sulfamethoxazole-trimethoprim, while 73.53% and 38.23% of them were sensitive for colistin sulfate and levofloxacin, respectively. Antibiotic resistance genes as
gene were tested for isolated
and detected by incidence rate of 91.18%.
resistance genes resembling as
and
genes were detected in isolated
.
70.6% and 14.7%, respectively.
.
isolated from chickens in Egypt was carried
and antibiotic-resistant genes that affect the poultry industry.