Corticosterone enhances N -methyl- d -aspartate receptor signaling to promote isolated ventral tegmental area activity in a reconstituted mesolimbic dopamine pathway

Highlights • Midbrain and striatal cultures functionally reintegrate synaptic contacts ex vivo. • Corticosterone causes glutamate receptor-dependent cell loss in midbrain neurons. • Ventral striatum is insensitive to effects of either corticosterone or an excitotoxin.

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Bibliographic Details
Published in:	Brain research bulletin Vol. 120; pp. 159 - 165
Main Authors:	Berry, Jennifer N, Saunders, Meredith A, Sharrett-Field, Lynda J, Reynolds, Anna R, Bardo, Michael T, Pauly, James R, Prendergast, Mark A
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-01-2016
Subjects:	Animals Antigens, Nuclear - metabolism Coculture Techniques Corticosterone Corticosterone - administration & dosage Corticosterone - metabolism Dopamine Female Male Mifepristone - pharmacology N-Methylaspartate - administration & dosage N-Methylaspartate - metabolism Nerve Tissue Proteins - metabolism Neurology Neurons - drug effects Neurons - metabolism Neurotransmitter Agents - pharmacology Organotypic Propidium - administration & dosage Propidium - metabolism Rats, Sprague-Dawley Receptors, Glucocorticoid - antagonists & inhibitors Receptors, Glucocorticoid - metabolism Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors Receptors, N-Methyl-D-Aspartate - metabolism Reward Stress Ventral Striatum - drug effects Ventral Striatum - metabolism Ventral Tegmental Area - drug effects Ventral Tegmental Area - metabolism glucocorticoid receptor VTA DMSO N-methyl- d-aspartate APV ACTH phosphate-buffered saline adrenocorticotropic hormone neuronal nuclear protein Ca 2 FITC dimethyl sulfoxide Organotypic analysis of variance minimum essential medium ANOVA propidium iodide corticotrophin releasing hormone NMDA DL-2-amino-7-phosphonovaleric acid PBS calcium Dopamine MR GR mineralocorticoid receptor Stress CORT NA MEM NeuN ventral tegmental area hypothalamus–pituitary–adrenal fluorescein isothiocynate CRH HPA PI ccnucleus accumbens Reward corticosterone Corticosterone Ca2
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Summary:	Highlights • Midbrain and striatal cultures functionally reintegrate synaptic contacts ex vivo. • Corticosterone causes glutamate receptor-dependent cell loss in midbrain neurons. • Ventral striatum is insensitive to effects of either corticosterone or an excitotoxin.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present Address: Jennifer N. Berry, Ph.D., Medicinal Chem. & Molecular Pharmacology Dept., Purdue University, 575 Stadium Mall Dr., RHPH 318, West Lafayette, IN 47907, Telephone: +1765-494-5003, Fax: +1765-494-1414, jnberry@purdue.edu
ISSN:	0361-9230 1873-2747
DOI:	10.1016/j.brainresbull.2015.11.018