Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry

• Published in: Analytical and bioanalytical chemistry Vol. 401; no. 5; pp. 1651 - 1659
• Main Authors: Alberici, Luciane C., Oliveira, Helena C. F., Catharino, Rodrigo R., Vercesi, Anibal E., Eberlin, Marcos N., Alberici, Rosana M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-09-2011; Springer
• Subjects: Analytical Chemistry; Animals; Arachidonic acid; Biochemistry; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fatty acids; Food Science; Humans; Hypertriglyceridemia - metabolism; Ionization; Laboratory Medicine; Lipid Metabolism; Lipids; Lipids - analysis; Lipids - chemistry; Liver; Liver - chemistry; Liver - metabolism; Mass spectrometry; Mice; Mice, Inbred C57BL; Mice, Transgenic; Monitoring/Environmental Analysis; Monounsaturated fatty acids; Original Paper; Other chromatographic methods; Spectrometric and optical methods; Spectrometry, Mass, Electrospray Ionization - methods; Triacylglycerols; Triglycerides; Triglycerides - analysis; Ambient ionization; Lipids; Mice liver; Hypertriglyceridemia; Mass spectrometry; Fourier transform spectroscopy; Liver; Free fatty acid; Arachidonic acid; Mixture; Tissue; Ex vivo; Oleic acid; Genetics; High performance; Use; Rodentia; Ion cyclotron resonance spectrometry; Positive ion; Thin layer chromatography; Vertebrata; Mammalia; Mouse; Ionization; Phosphatidylcholine; Negative ion
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-011-5208-8

Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps. Intense ions for phosphatidylcholines and triacylglycerols were observed in the positive ion mode whereas the spectra in the negative ion mode provided profiles of phosphatidylethanolamines and phosphatidylinositol. EASI-MS was coupled to high-performance thin-layer chromatography for analysis of free fatty acids. Fourier transform–ion cyclotron resonance–mass spectrometry was also employed to confirm the identity of the detected lipids. We demonstrated higher incorporation of oleic acid in phosphatidylcholine and triacylglycerol composition, higher relative abundance of arachidonic acid containing phosphatidylinositol, and overall distinct free fatty acid profile in the livers of genetic hypertriglyceridemic mice. We propose that these alterations in liver lipid composition are related to the higher tissue and body metabolic rates described in these hypertriglyceridemic mice.