Measurement of Phosphatidylcholine Hydroperoxide in Mild Ischemia-Reperfusion Injury in Rat Intestine

• Published in: The Tohoku Journal of Experimental Medicine Vol. 198; no. 4; pp. 223 - 232
• Main Authors: MASUKO, TSUYOSHI, FUNAYAMA, YUJI, NAITO, HIROO, FUKUSHIMA, KOHEI, SHIBATA, CHIKASHI, TAKAHASHI, KEN-ICHI, OGAWA, HITOSHI, UENO, TATSUYA, HASHIMOTO, AKIHIKO, MIYAZAWA, TERUO, NAKAGAWA, KIYOTAKA, MATSUNO, SEIKI, SASAKI, IWAO
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 01-12-2002
• Subjects: Animals; Disease Models, Animal; Immunohistochemistry; Intercellular Adhesion Molecule-1 - metabolism; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; intestine; ischemia-reperfusion injury; lipid peroxide; Male; Neutrophils - pathology; Phosphatidylcholines - metabolism; rat; Rats; Rats, Sprague-Dawley; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Sensitivity and Specificity; Thiobarbituric Acid Reactive Substances - metabolism; Time Factors
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.198.223

In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.