Effects of high-fat diet on plasma lipids, adiposity, and inflammatory markers in ovariectomized C57BL/6 mice

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 28; no. 3; pp. 316 - 323
• Main Authors: Ludgero-Correia, Antonio, B.Sc., M.Sc, Aguila, Marcia B., R.D., Ph.D, Mandarim-de-Lacerda, Carlos A., M.D., Ph.D, Faria, Tatiane S., B.Sc., Ph.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-03-2012; Elsevier; Elsevier Limited
• Subjects: Abdomen; Adipokines - metabolism; Adiponectin - blood; Adipose tissue; Adipose Tissue - metabolism; Adiposity - drug effects; Animals; Biochemical analysis; Biological and medical sciences; Biomarkers - blood; Body Weight; C57BL/6 mice; Cholesterol - blood; Cytokines; Diet; Diet, High-Fat - adverse effects; Dietary Fats - administration & dosage; Feeding. Feeding behavior; Female; Food; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; High-fat diet; Inflammation; Inflammation - etiology; Inflammation - pathology; Interleukin-6 - blood; Laboratory animals; Leptin - blood; Lipid Metabolism; Lipids; Menopause; Metabolic disorders; Mice; Mice, Inbred C57BL; Obesity; Obesity - etiology; Obesity - metabolism; Ovariectomy; Ovariectomy - adverse effects; Pathogens; Plasma; Rodents; Surgery; Triglycerides - blood; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Womens health; Brazil; Obesity; Inflammation; Ovariectomy; C57BL/6 mice; High-fat diet; Rodentia; Nutrition disorder; Lipids; Marker; Feeding; Blood plasma; Vertebrata; Mammalia; Adiposity; Diet; Mouse; Animal; Fat; Nutritional status
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2011.07.014

Abstract Objective We hypothesized that a high-fat (HF) diet aggravates ovariectomy-related complications. To test this hypothesis, ovariectomized (OVX) mice were fed a HF diet, and we investigated the lipid metabolism, adipose tissue remodeling, adipokines, and inflammatory cytokines. Methods To investigate the situation in a mouse model of ovariectomy, OVX and SHAM C57BL/6 mice fed a HF diet (60% fat) or standard chow (SC, 10% fat) were monitored for 18 wk. We evaluated daily food intake and weekly body weight. Mice were killed at 30 wk of age. Blood samples and adipose tissue were collected for biochemical, histologic, and molecular analysis. Results OVX groups showed atrophied uterus compared to the SHAM groups, ensuring the success of surgically induced menopause. Despite lower food intake, OVX-HF mice gained about 52% more weight and had heavier total body fats, especially in relation to ovarian fat pad (372%)—a visceral fat which is associated with increased pathogenicity in obesity, and showed larger adipocytes (30%) when compared to OVX-SC mice. Biochemical analysis showed that the OVX-HF mice had increased levels of serum total cholesterol (51%), greater serum triglycerides (158%), lower serum adiponectin (40%), and higher plasma leptin (323%) than OVX-SC mice. The obese group (OVX-HF) also had higher IL-6 levels than both SHAM-HF (241%) and OVX-SC mice (870%). Conclusion OVX C57BL/6 mice fed HF diet had greater adipose fat pad, larger adipocytes, and increased inflammatory markers, reinforcing the idea that a HF diet aggravates the complications of ovariectomy-associated inflammation.