Excretory-secretory products from the brown stomach worm, Teladorsagia circumcincta, exert antimicrobial activity in in vitro growth assays

• Published in: Parasites & vectors Vol. 15; no. 1; pp. 1 - 354
• Main Authors: Rooney, James, Williams, Timothy L, Northcote, Holly M, Frankl, Fiona E. Karet, Price, Daniel R. G, Nisbet, Alasdair J, Morphew, Russell M, Cantacessi, Cinzia
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 02-10-2022; BioMed Central; BMC
• Subjects: Animals; Antibacterial agents; Antigens; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Antimicrobial peptide; Antimicrobial peptides; Assaying; Bacteria; Biofilms; Biological products; Chemical properties; Chromatography; Colonies; Data points; Depletion; Disease resistance; E coli; Electron microscopy; Escherichia coli; Extracellular vesicle; Extracellular vesicles; Gastroenteritis; Gastrointestinal helminth; Growth; Identification and classification; Infections; Intestinal flora; Intestinal microflora; Liquid chromatography; Livestock; Mass spectrometry; Mass spectroscopy; Microbiological research; Microbiome; Microbiomes; Microbiota; Nanoparticles; Nematoda; Parasites; Peptides; Physiological aspects; Proteins; Proteomics; Ruminant; Secretory products; Size exclusion chromatography; Stomach; Teladorsagia circumcincta; Tracking; Transmission electron microscopy; United Kingdom; United States > US
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-022-05443-z

Over the past decade, evidence has emerged of the ability of gastrointestinal (GI) helminth parasites to alter the composition of the host gut microbiome; however, the mechanism(s) underpinning such interactions remain unclear. In the current study, we (i) undertake proteomic analyses of the excretory-secretory products (ESPs), including secreted extracellular vesicles (EVs), of the 'brown stomach worm' Teladorsagia circumcincta, one of the major agents causing parasite gastroenteritis in temperate areas worldwide; (ii) conduct bioinformatic analyses to identify and characterise antimicrobial peptides (AMPs) with putative antimicrobial activity; and (iii) assess the bactericidal and/or bacteriostatic properties of T. circumcincta EVs, and whole and EV-depleted ESPs, using bacterial growth inhibition assays. Size-exclusion chromatography was applied to the isolation of EVs from whole T. circumcincta ESPs, followed by EV characterisation via nanoparticle tracking analysis and transmission electron microscopy. Proteomic analysis of EVs and EV-depleted ESPs was conducted using liquid chromatography-tandem mass spectrometry, and prediction of putative AMPs was performed using available online tools. The antimicrobial activities of T. circumcincta EVs and of whole and EV-depleted ESPs against Escherichia coli were evaluated using bacterial growth inhibition assays. Several molecules with putative antimicrobial activity were identified in both EVs and EV-depleted ESPs from adult T. circumcincta. Whilst exposure of E. coli to whole ESPs resulted in a significant reduction of colony-forming units over 3 h, bacterial growth was not reduced following exposure to worm EVs or EV-depleted ESPs. Our data points towards a bactericidal and/or bacteriostatic function of T. circumcincta ESPs, likely mediated by molecules with antimicrobial activity.