Pentoxifylline/Chitosan Films on Wound Healing: In Vitro/In Vivo Evaluation

• Published in: Pharmaceutics Vol. 15; no. 4; p. 1122
• Main Authors: Moreira, Vandiara Martins, Leite, Joandra Maísa da Silva, Medeiros, Kaline de Araújo, Assis, Karoll Moangella Andrade de, Borges, Joyce Cordeiro, Santana, Lucas Matheus Barreto, Moreira, Lívia Maria Coelho de Carvalho, Alves, Larissa Pereira, Oliveira, Tharcia Kiara Beserra de, Silveira, João Walter de Souza da, Silva, Dayanne Tomaz Casimiro da, Damasceno, Bolívar Ponciano Goulart de Lima
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31-03-2023; MDPI
• Subjects: Bandages and bandaging; Biopolymers; Chemical properties; Chitin; chitosan; dressings; Health aspects; Materials; Pentoxifylline; Pharmaceutical research; Spectrum analysis; Tumor necrosis factor-TNF; Wound healing; Brazil; United States > US; Japan; chitosan; dressings; films; pentoxifylline; wound healing
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics15041122

This study aimed to develop films of chitosan (CSF) associated with pentoxifylline (PTX) for healing cutaneous wounds. These films were prepared at two concentrations, F1 (2.0 mg/mL) and F2 (4.0 mg/mL), and the interactions between the materials, structural characteristics, in vitro release, and morphometric aspects of skin wounds in vivo were evaluated. The formation of the CSF film with acetic acid modifies the polymeric structure, and the PTX demonstrates interaction with the CSF, in a semi-crystalline structure, for all concentrations. The release for all films was proportional to the concentration, with two phases: a fast one of ≤2 h and a slow one of >2 h, releasing 82.72 and 88.46% of the drug after 72 h, being governed by the Fickian diffusion mechanism. The wounds of the mice demonstrate a reduction of up to 60% in the area on day 2 for F2 when compared to CSF, F1, and positive control, and this characteristic of faster healing speed for F2 continues until the ninth day with wound reduction of 85%, 82%, and 90% for CSF, F1, and F2, respectively. Therefore, the combination of CSF and PTX is effective in their formation and incorporation, demonstrating that a higher concentration of PTX accelerates skin-wound reduction.