Isolation, expansion and characterisation of mesenchymal stem cells from human bone marrow, adipose tissue, umbilical cord blood and matrix: a comparative study

• Published in: Cytotechnology (Dordrecht) Vol. 67; no. 5; pp. 793 - 807
• Main Authors: Secunda, R., Vennila, Rosy, Mohanashankar, A. M., Rajasundari, M., Jeswanth, S., Surendran, R.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2015; Springer Nature B.V
• Subjects: Adipose tissue; Antibodies; Biochemistry; Biomedicine; Biotechnology; Body fat; Bone marrow; CD105 antigen; CD14 antigen; CD29 antigen; CD34 antigen; CD44 antigen; CD45 antigen; CD73 antigen; Cell growth; Chemistry; Chemistry and Materials Science; Cord blood; Flow cytometry; Liver diseases; Mesenchymal stem cells; Original Research; Penicillin; Stem cells; Umbilical cord; United States > US; India; Oct-4; Stem cell therapy; Explant method; Mesenchymal stem cells
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-014-9718-z

The multipotent and immunosuppressive capacities of mesenchymal stem cells (MSCs) attract several scientists worldwide towards translational research focusing on treatment of diseases including liver failure. Though MSC’s have been isolated from different sources, researchers do not concur on the best source for expansion and clinical translation. In this study, we have compared the isolation, proliferation and expansion of MSCs from umbilical cord blood (UCB), Wharton’s Jelly (WJ), bone marrow (BM) and adipose tissue (AT). MSCs were isolated by density gradient separation from UCB, BM and AT and by both enzymatic and explant method for WJ. The MSCs are characterized by their ability to adhere to plastic, expression of positive (CD105, CD73, CD90, CD29, CD44) and negative (CD45, CD14, CD34) markers by flow cytometry and also by their in vitro adipogenic, osteogenic and chondrogenic differentiation. This comprehensive study clearly shows that WJ is better than UCB both in terms of rapidity, yield and ease of procedure. AT and BM are autologous sources for MSC’s but the specimen collection involves cumbersome and painful procedures and an invasive approach. However being autologous, they are safe and probable candidates for therapeutic future applications.